COL1A2 Antibody (E-6) recommended for detection of Collagen α2 Type I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

COL1A2 Antibody (E-6): sc-393573

COL1A2 Antibody (E-6) is rated 4.6 out of 5 by 15.
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Datasheets

    • Anti-COL1A2 Antibody (E-6) is a mouse monoclonal IgG1 (lambda light chain) COL1A2 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1064-1093 at the C-terminus of Collagen α2 Type I of mouse origin
    • Anti-COL1A2 Antibody (E-6) is recommended for detection of Collagen α2 Type I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-COL1A2 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-393573 P
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COL1A2 (E-6): sc-393573.
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マウスの組織で、WBとIFあるいはIHCを考えています。 CollagenⅠ A1との交差反応が見られるのでしょうか。 あるいはA1は染まらず、A2のみ特異的に検出できるのでしょうか。

Asked by: Tom0112
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

To my question about IHC", you mentioned the product COL1A2 (E-6): sc-393573. But the description of the product suggest "HRP (sc-393573 HRP)" for IHC(P). Is there no difference between the products "sc-393573" and "sc-393573 HRP" for IHC(P) and WB?

Asked by: David27
Thank you for your question. The difference between the catalog numbers, sc-393573 and sc-393573 HRP, is that the latter is the primary antibody directly conjugated to horse radish peroxidase (HRP). This antibody is suitable for WB and IHC(P), and if you purchase the unconjugated antibody, you will need to use a secondary antibody, or mouse IgG binding protein for detection. Alternatively, if you prefer to use a directly conjugated primary antibody, you can purchase the HRP conjugated antibody and also use it for both WB and IHC(P).
Answered by: Technical Support
Date published: 2018-04-09

Also, what is the recommended secondary antibody in IHC? There are only those written for IF, WB etc

Asked by: David27
Thank you for your question. We would recommend using our HRP-conjugated mouse IgG binding protein, m-IgGκ BP-HRP (sc-516102), for indirect immunoperoxidase staining, in lieu of conventional anti-mouse secondary antibodies. Alternatively, we would recommend if you are using the Avidin-Biotin Complex (ABC) staining protocol to select any conventional biotinylated anti-mouse IgG secondary antibody and use in conjunction with our ImmunoCruz® ABC Kit (sc-516216).
Answered by: Technical Support
Date published: 2017-07-20

What is the recommended agent for antigen unmasking in IHC

Asked by: David27
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval methods using this antibody, COL1A2 (E-6): sc-393573. A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-07-20

What application is the blocking peptide sc-393573 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 15
Date published: 2017-01-18
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Rated 3 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and gave a satisfactory signal at dilution 1:25
Date published: 2018-11-19
Rated 4 out of 5 by from COL1A2 Antibody (E-6) COL1A2 (E-6): sc. Near-infrared western blot analysis of LDH-A expression in MEF whole cell lysates. Blocked with 5% NFDM for 1 hour. Detection reagent used: DAM CW800 (Licor).
Date published: 2018-06-28
Rated 5 out of 5 by from 抗体很不错 抗体很好用,特异性好,就是效价低了点,其他都不错,Santa的抗体就是很好用,不错的选择,还送优惠券
Date published: 2018-04-28
Rated 5 out of 5 by from great antibody It is great for western blot and staining for mouse heart sample.
Date published: 2017-12-14
Rated 4 out of 5 by from Good antibody for IF I Used 1:200 dilution in paraffined mouse kidney. I have got good IF photos.
Date published: 2017-08-28
Rated 4 out of 5 by from good for WB I use 1:500 dilution and get clear band at 130kd, but have some undesired bands
Date published: 2017-08-18
Rated 5 out of 5 by from The antibody is good This antibody is very sutiable for WB, it shows a very clear band.
Date published: 2017-07-25
Rated 5 out of 5 by from col1a2 antibody I have tried the col1a2 antibody in Western blotting, there is a very clear and strong band in approximately 130kDa, the background is so clear and the antibody is nice for WB.
Date published: 2017-05-23
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