COL1A1 Antibody (C-18): sc-8784

Thank you for your question. What dilution we use depends on the application it is being used for, as well as sample type and secondary reagents, but here is a general guideline of starting dilutions (further titration/optimization will be required): Western blot: 1:100 Immunofluorescence/IHC: 1:50 Most other applications, we recommend doing a titration.

Thank you for your question. Which blocking reagent depends on which application you are using the antibody for. For Western blot we recommend 5% milk with TBS-T. For IHC and IF applications we recommend using blocking serum derived from the same species in which the secondary antibody is raised.