Thank you for your question. What dilution we use depends on the application it is being used for, as well as sample type and secondary reagents, but here is a general guideline of starting dilutions (further titration/optimization will be required):
Western blot: 1:100
Immunofluorescence/IHC: 1:50
Most other applications, we recommend doing a titration.
Answered by: Tech Service
Date published: 2022-09-27