CNT1 Antibody (G-7): sc-515874

CNT1 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects CNT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CNT1 (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CNT1 protein, also known as solute carrier family 28 (sodium-coupled nucleoside transporter), member 1 (SLC28A1), plays a crucial role in the transport of nucleosides across the plasma membrane, which is essential for cellular signaling and metabolism. Specifically, CNT1 facilitates the sodium-dependent uptake of uridine, azidodeoxythymidine (AZT), and adenosine, while not transporting guanosine or deoxyadenosine, which are instead secreted by the kidneys. This selectivity suggests CNT1 may be involved in the renal reabsorption of physiologic nucleosides and synthetic nucleoside drugs, thereby influencing therapeutic outcomes in the treatment of neoplastic and viral diseases. The deduced human CNT1 protein consists of 650 amino acids and exhibits 83% identity with the rat counterpart, with expression primarily in the intestine, kidney, and liver, highlighting CNT1′s importance in both nutrient absorption and drug pharmacokinetics.

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CNT1 Antibody (G-7) References:

1. Developmental regulation of the concentrative nucleoside transporters CNT1 and CNT2 in rat liver.  |  del Santo, B., et al. 2001. J Hepatol. 34: 873-80. PMID: 11451171
2. Functional and genetic diversity in the concentrative nucleoside transporter, CNT1, in human populations.  |  Gray, JH., et al. 2004. Mol Pharmacol. 65: 512-9. PMID: 14978229
3. Conserved residues F316 and G476 in the concentrative nucleoside transporter 1 (hCNT1) affect guanosine sensitivity and membrane expression, respectively.  |  Lai, Y., et al. 2005. Am J Physiol Cell Physiol. 288: C39-45. PMID: 15456697
4. Interaction of nucleoside inhibitors of HIV-1 reverse transcriptase with the concentrative nucleoside transporter-1 (SLC28A1).  |  Cano-Soldado, P., et al. 2004. Antivir Ther. 9: 993-1002. PMID: 15651758
5. The concentrative nucleoside transporter family (SLC28): new roles beyond salvage?  |  Aymerich, I., et al. 2005. Biochem Soc Trans. 33: 216-9. PMID: 15667311
6. Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.  |  Rodríguez-Mulero, S., et al. 2005. Kidney Int. 68: 665-72. PMID: 16014043
7. Different signaling pathways utilized by insulin to regulate the expression of ENT2, CNT1, CNT2 nucleoside transporters in rat cardiac fibroblasts.  |  Podgorska, M., et al. 2007. Arch Biochem Biophys. 464: 344-9. PMID: 17537394
8. Polymorphisms in ABCG2, ABCC3 and CNT1 genes and their possible impact on chemotherapy outcome of lung cancer patients.  |  Müller, PJ., et al. 2009. Int J Cancer. 124: 1669-74. PMID: 19107936
9. Hepatocyte nuclear factor-4alpha and bile acids regulate human concentrative nucleoside transporter-1 gene expression.  |  Klein, K., et al. 2009. Am J Physiol Gastrointest Liver Physiol. 296: G936-47. PMID: 19228884
10. Contribution of CNT1 and ENT1 to ribavirin uptake in human hepatocytes.  |  Choi, MK., et al. 2015. Arch Pharm Res. 38: 904-13. PMID: 25011570
11. Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.  |  Yamamura, T., et al. 2021. Biochem Biophys Res Commun. 558: 120-125. PMID: 33910126
12. Increased renal elimination of endogenous and synthetic pyrimidine nucleosides in concentrative nucleoside transporter 1 deficient mice.  |  Persaud, AK., et al. 2023. Nat Commun. 14: 3175. PMID: 37264059

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CNT1 Antibody (G-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CNT1 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CNT1 Antibody (G-7) Specifications

Alternate Product Name: CNT1

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-70

Epitope Target: CNT1

Epitope Species: human

Product Reactivity/Detection: CNT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CNT1 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin