CNPase Antibody (B-1): sc-166019

CNPase Antibody (B-1) is a mouse monoclonal IgG2b antibody that detects 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CNPase is a crucial membrane-bound enzyme that plays a significant role in linking tubulin to membranes and regulating the distribution of cytoplasmic microtubules, which is vital for maintaining cellular structure and function. CNPase acts as a microtubule-associated protein, promoting microtubule assembly, particularly through its C-terminal region. CNPase is predominantly found in the central nervous system, where CNPase is highly concentrated in myelin, as well as in the outer segments of photoreceptors in the retina, highlighting CNPase′s importance in neuronal health and visual function. Notably, disturbances in CNPase activity, such as those caused by acute lead intoxication, can lead to significant alterations in myelin morphology, underscoring CNPase′s critical role in neuroprotection and myelination. Anti-CNPase antibody (B-1) is an essential tool for researchers studying the dynamics of microtubule organization and the implications of CNPase in various neurological conditions.

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CNPase Antibody (B-1) References:

1. Acute lead intoxication in vivo affects myelin membrane morphology and CNPase activity.  |  Dabrowska-Bouta, B., et al. 2000. Exp Toxicol Pathol. 52: 257-63. PMID: 10930127
2. Expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and its roles in activated microglia in vivo and in vitro.  |  Yang, L., et al. 2014. J Neuroinflammation. 11: 148. PMID: 25148928
3. Administration of vitamin D3 induces CNPase and myelin oligodendrocyte glycoprotein expression in the cerebral cortex of the murine model of cuprizone-induced demyelination.  |  Mashayekhi, F. and Salehi, Z. 2016. Folia Neuropathol. 54: 259-264. PMID: 27764518
4. Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase.  |  Muruganandam, G., et al. 2017. BMC Biochem. 18: 7. PMID: 28511668
5. Supplemental study on 2', 3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNPase) activity in developing rat spinal cord lesions induced by hexachlorophene and cuprizone.  |  Kanno, T., et al. 2019. J Vet Med Sci. 81: 1368-1372. PMID: 31447458
6. Sirt3 increases CNPase enzymatic activity through deacetylation and facilitating substrate accessibility.  |  Wang, D., et al. 2021. Biochem Biophys Res Commun. 571: 181-187. PMID: 34330062
7. CNPase, a 2',3'-Cyclic-nucleotide 3'-phosphodiesterase, as a Therapeutic Target to Attenuate Cardiac Hypertrophy by Enhancing Mitochondrial Energy Production.  |  Tan, KS., et al. 2021. Int J Mol Sci. 22: PMID: 34639145
8. EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone.  |  Lira-Diaz, E., et al. 2022. Life (Basel). 12: PMID: 35330085
9. Normal pressure hydrocephalus decreases the proliferation of oligodendrocyte progenitor cells and the expression of CNPase and MOG proteins in the corpus callosum before behavioral deficits occur.  |  Campos-Ordoñez, T., et al. 2023. Exp Neurol. 365: 114412. PMID: 37075967
10. Nanobodies against the myelin enzyme CNPase as tools for structural and functional studies.  |  Markusson, S., et al. 2025. J Neurochem. 169: e16274. PMID: 39655780

The preferred CNPase antibody is CNPase Antibody (H-2): sc-166558.