Clusterin Antibody (A-9): sc-166907

Clusterin Antibody (A-9) is a mouse monoclonal IgG2a kappa light chain antibody designed to recognize full-length native Clusterin of human origin by targeting an epitope mapped between amino acids 120-449 at the C-terminus. Anti-Clusterin antibody (A-9) is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), ensuring reliable detection of Clusterin in human samples. Clusterin, also known as apolipoprotein J (ApoJ) or complement lysis inhibitor (CLI), is a multifunctional secretory heterodimeric glycoprotein involved in immune regulation, cell adhesion, lipid transportation, tissue remodeling, membrane recycling, and cell-cell interactions. Within cells, Clusterin is predominantly localized in the extracellular matrix, where Clusterin plays a critical role in maintaining cellular homeostasis by inhibiting the complement system and preventing unwanted immune responses. Additionally, Clusterin is found in the cytoplasm and nucleus, where Clusterin is involved in regulating apoptosis, protecting cells from stress-induced damage, and facilitating intracellular signaling pathways. This strategic localization is essential for diverse functions, allowing Clusterin to interact with various proteins and cellular structures to modulate processes such as cell survival, inflammation, and tissue repair. The ability to shuttle between different cellular compartments enables Clusterin to perform protective roles both extracellularly and intracellularly, thereby maintaining cellular integrity and responding to physiological stressors. By specifically targeting the C-terminal region, Clusterin monoclonal antibody (A-9) facilitates detailed studies of distribution and interactions within different cellular environments, providing valuable insights into functional mechanisms and involvement in pathological conditions such as cancer, where Clusterin is often overexpressed to support tumor progression and metastasis, and neurodegenerative diseases like Alzheimer′s disease, where Clusterin influences amyloid-beta aggregation and neuronal toxicity. Whether employed in basic research to elucidate molecular pathways or in clinical diagnostics to assess expression and localization in patient samples, Clusterin (A-9) antibody offers reliable and precise detection, thereby supporting advancements in understanding immune regulation, cancer biology, neurodegeneration, and the development of targeted therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Clusterin Antibody (A-9) References:

1. Apolipoprotein J/clusterin prevents a progressive glomerulopathy of aging.  |  Rosenberg, ME., et al. 2002. Mol Cell Biol. 22: 1893-902. PMID: 11865066
2. Synthesis and functional analyses of nuclear clusterin, a cell death protein.  |  Leskov, KS., et al. 2003. J Biol Chem. 278: 11590-600. PMID: 12551933
3. Clusterin as a biomarker in murine and human intestinal neoplasia.  |  Chen, X., et al. 2003. Proc Natl Acad Sci U S A. 100: 9530-5. PMID: 12886021
4. Modulation of different clusterin isoforms in human colon tumorigenesis.  |  Pucci, S., et al. 2004. Oncogene. 23: 2298-304. PMID: 14755245
5. Expression of clusterin in Müller cells of the rat retina after pressure-induced ischemia.  |  Gwon, JS., et al. 2004. Glia. 47: 35-45. PMID: 15139011
6. Up-regulated expression of cytoplasmic clusterin in human ovarian carcinoma.  |  Xie, D., et al. 2005. Cancer. 103: 277-83. PMID: 15578711
7. Delayed activation of insulin-like growth factor-1 receptor/Src/MAPK/Egr-1 signaling regulates clusterin expression, a pro-survival factor.  |  Criswell, T., et al. 2005. J Biol Chem. 280: 14212-21. PMID: 15689620
8. Clusterin inhibits apoptosis by interacting with activated Bax.  |  Zhang, H., et al. 2005. Nat Cell Biol. 7: 909-15. PMID: 16113678
9. Apolipoprotein J: structure and tissue distribution.  |  de Silva, HV., et al. 1990. Biochemistry. 29: 5380-9. PMID: 1974459

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Clusterin Antibody (A-9) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-Clusterin Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Clusterin Antibody (A-9) Specifications

Alternate Product Name: Clusterin

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 120-449

Epitope Target: Clusterin

Epitope Species: human

Product Reactivity/Detection: Clusterin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for Clusterin Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Clusterin-α antibody is Clusterin-α Antibody (B-5): sc-5289.