Anti-Clusterin-alpha Antibody (B-5) recommended for detection of Clusterin-α of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Clusterin-α Antibody (B-5): sc-5289

Anti-Clusterin-α Antibody (B-5) is rated 4.8 out of 5 by 10.
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Datasheets

    • Anti-Clusterin-α Antibody (B-5) is a mouse monoclonal IgG1 κ Clusterin-α antibody, cited in 42 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 432-449 at the C-terminus of Clusterin-α of human origin
    • Clusterin-alpha Antibody (B-5) is recommended for detection of Clusterin-α of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Clusterin-alpha Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Clusterin-α (B-5): sc-5289.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Clusterin-α Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Clusterin-α Antibody (B-5) (see ordering information below).
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whether this antibody could be used for mouse tissues.

Asked by: Anonymous
Thank you for your question. Clusterin-alpha Antibody (B-5) is only recommended for detection of Clusterin-α of human origin by WB, IP, IF, IHC(P) and ELISA. It is not suitable for cell or tissue samples from mouse.
Answered by: Tech Support Europe
Date published: 2021-01-27

The protein band detected as clusterin-alpha percursor corresponds to the percursor containing both the N-signal and alpha and beta sequences right?

Asked by: jvsilva
Thank you for your question. Clusterin-α Antibody (B-5): sc-5289 recognizes amino acids 432-449, which correspond to Clusterin alpha chain. As such, the antibody detects any form of Clusterin containing this sequence.
Answered by: Technical Support Europe
Date published: 2020-10-16

I want to ask why many clusterin antibody are clusterin-alpha chain antibody? not alpha and beta chain together, or only beta chain? Does it really can identify clusterin protein? Don't know how to choose the suitable clusterin antibody.

Asked by: Rachelwong
Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, α and β, are associated through disulfide bonds. The β subunit (also called ApoJα) corresponds to residues 23-227. The α subunit (also called ApoJβ) corresponds to residues 228-449. Based on epitope mapping of the antibody, it is possible to make antibodies that are specific to either the alpha or beta chain. If the epitope has not been mapped, then the antibodies may be recommended for detection of Clusterin protein.
Answered by: Technical Support
Date published: 2018-12-11

hello, i want to detect to nuclear clusterin as IHC-P and WB. So can i use Clusterin-α Antibody (B-5): sc-5289 ? Because i see H-330 doesent product anymore.

Asked by: histolog
Thank you for your question. Yes, our mouse monoclonal antibody, Clusterin-α (B-5): sc-5289, is recommended for detection of Clusterin-α of human origin by WB, IP, IF, IHC(P) and ELISA. The rabbit polyclonal (H-330) antibody has been discontinued, and this is the suggested replacement. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department to request a sample. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-30

How should the blocking peptide sc-5289 P be used?

Asked by: jenni
Thank you for your question. The blocking peptide is intended to be used as a negative control by preadsorbing the Clusterin-α (B-5): sc-5289 with it prior to application. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 13
Date published: 2017-01-20

How can Clusterin-α (B-5): sc-5289 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Clusterin-α (B-5): sc-5289 monoclonal antibody is currently available conjugated to phycoerythrin (sc-5289 PE), fluorescein (sc-5289 FITC), Alexa Fluor® 488 (sc-5289 AF488) or Alexa Fluor® 647 (sc-5289 AF647)
Answered by: Technical Support 9
Date published: 2017-01-16
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Rated 4 out of 5 by from Good specificity Worked great for a sample that had a very small protein concentration
Date published: 2019-08-21
Rated 5 out of 5 by from good staining Good nuclear signal in immunostaining of embryonic cardiomyocytes (mouse).
Date published: 2017-02-25
Rated 5 out of 5 by from Great Clusterin-alpha specificity Great antibody with known specificity to Clusterin-alpha through siRNA knockdown data. Strong staining in IHC and robust bands in WB.
Date published: 2017-01-30
Rated 5 out of 5 by from Great WB and IHC Outstanding product; take a look at IHC staining data; as good as it gets !!
Date published: 2017-01-26
Rated 4 out of 5 by from Positive detection of both the precursor Positive detection of both the precursor and mature forms of the Clusterin- protein by WB using SK-BR-3 whole cell lysate. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Clusterin- protein via Western Blot analysis. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Publishable data obtained by IHC using FFPE Publishable data obtained by IHC using FFPE human eye tissue. -SCBT Publication Review
Date published: 2015-01-26
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