Anti-Clusterin-alpha Antibody (A-11) recommended for detection of Clusterin-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Clusterin-α Antibody (A-11): sc-166831

Anti-Clusterin-α Antibody (A-11) is rated 5.0 out of 5 by 3.
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Datasheets

    • Clusterin-α Antibody (A-11) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 432-449 at the C-terminus of Clusterin-α of human origin
    • Anti-Clusterin-alpha Antibody (A-11) is recommended for detection of Clusterin-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Clusterin-alpha Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Clusterin-α (A-11): sc-166831.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Clusterin-α Antibody (A-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Clusterin-α Antibody (A-11) (see ordering information below).
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Hi, since alpha chain of clusterin=ApoJ-beta, and beta chain=APoJ-alpha, then which end of the protein does the A-11 antibody called clusterin-alpha detects? Does it react with N or C -terminal fragment of the protein?

Asked by: jackie27111
Thank you for your question. This antibody is specific for an epitope mapping between amino acids 420-449 at the C-terminus of Clusterin-α of human origin (accession#P10909).
Answered by: Tech Service
Date published: 2019-12-06

We need to test expression of equine clusterin. We will much appreciate if you send us a sample of this and other anticlusterin Ab to test. Once we confirm the cross reactivity (WIB, IHQ) we can give you validation and purchase the one of best results.

Asked by: MonicaVL
Thank you for your question. Please contact your local distributor or information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

What application is the blocking peptide sc-166831 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of Clusterin-α expression in A549, SK-BR-3 and PANC-1 whole cell lysates.
Date published: 2017-02-08
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Nice Western Blot data of Clusterin expression Nice Western Blot data of Clusterin expression in non-transfected and human Clusterin transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-11-17
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