CLS1 Antibody (E-8): sc-514986
- CLS1 Antibody (E-8) is a mouse monoclonal IgG1 κ CLS1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 88-301 mapping at the C-terminus of CLS1 of human origin
- CLS1 Antibody (E-8) is recommended for detection of CLS1 of human origin by WB, IP, IF and ELISA
- Anti-CLS1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CLS1 Antibody (E-8) for WB applications. These reagents are now offered in bundles with CLS1 Antibody (E-8) (see ordering information below).
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CLS1 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLS1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLS1 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLS1, also known as cardiolipin synthetase, is a crucial mitochondrial protein that belongs to the CDP-alcohol phosphatidyltransferase class-I family. CLS1 functions as a multi-pass membrane protein located in the inner mitochondrial membrane, where CLS1 plays a vital role in the synthesis of cardiolipin, a unique phospholipid essential for mitochondrial function. Cardiolipin is characterized by having four fatty acid tails instead of the usual two, making cardiolipin a double phospholipid that constitutes approximately 20% of total mitochondrial lipids. This lipid maintains the integrity of the mitochondrial membrane and participates in various processes, including mitochondrial dynamics, energy production, and apoptosis. Defects in the CRLS1 gene can disrupt cardiolipin synthesis, leading to impaired metabolism and ATP production, which may contribute to conditions such as Barth syndrome. CLS1 (E-8) monoclonal antibody serves as an invaluable tool for researchers studying mitochondrial function and related metabolic disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLS1 Antibody (E-8) References:
- Expression of monolysocardiolipin acyltransferase activity is regulated in concert with the level of cardiolipin and cardiolipin biosynthesis in the mammalian heart. | Taylor, WA., et al. 2002. BMC Biochem. 3: 9. PMID: 12019031
- Absence of cardiolipin results in temperature sensitivity, respiratory defects, and mitochondrial DNA instability independent of pet56. | Zhong, Q., et al. 2004. J Biol Chem. 279: 32294-300. PMID: 15169766
- Cloning and characterization of a cDNA encoding human cardiolipin synthase (hCLS1). | Lu, B., et al. 2006. J Lipid Res. 47: 1140-5. PMID: 16547353
- Identification and characterization of human cardiolipin synthase. | Houtkooper, RH., et al. 2006. FEBS Lett. 580: 3059-64. PMID: 16678169
- Identification and functional characterization of hCLS1, a human cardiolipin synthase localized in mitochondria. | Chen, D., et al. 2006. Biochem J. 398: 169-76. PMID: 16716149
- Cardiolipin deficiency releases cytochrome c from the inner mitochondrial membrane and accelerates stimuli-elicited apoptosis. | Choi, SY., et al. 2007. Cell Death Differ. 14: 597-606. PMID: 16888643
- Compensatory role of the cis-trans-isomerase and cardiolipin synthase in the membrane fluidity of Pseudomonas putida DOT-T1E. | Bernal, P., et al. 2007. Environ Microbiol. 9: 1658-64. PMID: 17564601
- Cardiolipin synthesis for the assembly of bacterial and mitochondrial membranes. | Schlame, M. 2008. J Lipid Res. 49: 1607-20. PMID: 18077827
- Cardiolipin, a lipid found in mitochondria, hydrogenosomes and bacteria was not detected in Giardia lamblia. | Rosa, Ide A., et al. 2008. Exp Parasitol. 120: 215-20. PMID: 18691575
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLS1 Antibody (E-8) | sc-514986 | 200 µg/ml | $316.00 | |||
CLS1 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-531255 | 200 µg Ab; 10 µg BP | $354.00 | |||
CLS1 Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-524891 | 200 µg Ab, 40 µg BP | $354.00 | |||
CLS1 Antibody (E-8) AC | sc-514986 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CLS1 Antibody (E-8) HRP | sc-514986 HRP | 200 µg/ml | $316.00 | |||
CLS1 Antibody (E-8) FITC | sc-514986 FITC | 200 µg/ml | $330.00 | |||
CLS1 Antibody (E-8) PE | sc-514986 PE | 200 µg/ml | $343.00 | |||
CLS1 Antibody (E-8) Alexa Fluor® 488 | sc-514986 AF488 | 200 µg/ml | $357.00 | |||
CLS1 Antibody (E-8) Alexa Fluor® 546 | sc-514986 AF546 | 200 µg/ml | $357.00 | |||
CLS1 Antibody (E-8) Alexa Fluor® 594 | sc-514986 AF594 | 200 µg/ml | $357.00 | |||
CLS1 Antibody (E-8) Alexa Fluor® 647 | sc-514986 AF647 | 200 µg/ml | $357.00 | |||
CLS1 Antibody (E-8) Alexa Fluor® 680 | sc-514986 AF680 | 200 µg/ml | $357.00 | |||
CLS1 Antibody (E-8) Alexa Fluor® 790 | sc-514986 AF790 | 200 µg/ml | $357.00 |