CLK3 Antibody (D-10): sc-365225
- CLK3 Antibody (D-10) is a mouse monoclonal IgG1 κ CLK3 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 211-241 within an internal region of CLK3 of human origin
- CLK3 Antibody (D-10) is recommended for detection of CLK3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CLK3 Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CLK3 Antibody (D-10) for WB and IHC(P) applications. These reagents are now offered in bundles with CLK3 Antibody (D-10) (see ordering information below).
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CLK3 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLK3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CLK3 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLK3, also known as CDC-like kinase 3 or PHCLK3, is a 638 amino acid protein that plays a crucial role in the regulation of RNA splicing by acting as a dual-specificity kinase. CLK3 catalyzes the ATP-dependent phosphorylation of serine- and arginine-rich splicing factor proteins, which is vital for their functional modulation and proper localization within the nucleus. This phosphorylation process is essential for the regulation of gene expression and the maintenance of cellular homeostasis, as CLK3 influences the splicing of pre-mRNA into mature mRNA, thereby impacting protein diversity and function. CLK3 is part of a complex network of serine/threonine protein kinases that coordinate various cellular processes, including cell division and apoptosis, highlighting CLK3′s importance in cellular signaling pathways. Additionally, four isoforms of CLK3 arise from alternative splicing, further diversifying CLK3′s functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLK3 Antibody (D-10) References:
- The dual specificity protein kinase CLK3 is abundantly expressed in mature mouse spermatozoa. | Menegay, H., et al. 1999. Exp Cell Res. 253: 463-73. PMID: 10585269
- Protein kinase clk/STY is differentially regulated during erythroleukemia cell differentiation: a bias toward the skipped splice variant characterizes postcommitment stages. | García-Sacristán, A., et al. 2005. Cell Res. 15: 495-503. PMID: 16045812
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. | Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
- Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. | Wissing, J., et al. 2007. Mol Cell Proteomics. 6: 537-47. PMID: 17192257
- cDNA cloning and characterization of rat Clk3, a LAMMER kinase predominately expressed in testis. | Becker, W., et al. 1996. Biochim Biophys Acta. 1312: 63-7. PMID: 8679717
- The Clk2 and Clk3 dual-specificity protein kinases regulate the intranuclear distribution of SR proteins and influence pre-mRNA splicing. | Duncan, PI., et al. 1998. Exp Cell Res. 241: 300-8. PMID: 9637771
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLK3 Antibody (D-10) | sc-365225 | 200 µg/ml | $322.00 | |||
CLK3 Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-527343 | 200 µg Ab; 10 µg BP | $361.00 | |||
CLK3 Antibody (D-10): m-IgG1 BP-HRP Bundle | sc-532716 | 200 µg Ab; 20 µg BP | $361.00 | |||
CLK3 Antibody (D-10) AC | sc-365225 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CLK3 Antibody (D-10) HRP | sc-365225 HRP | 200 µg/ml | $322.00 | |||
CLK3 Antibody (D-10) FITC | sc-365225 FITC | 200 µg/ml | $336.00 | |||
CLK3 Antibody (D-10) PE | sc-365225 PE | 200 µg/ml | $349.00 | |||
CLK3 Antibody (D-10) Alexa Fluor® 488 | sc-365225 AF488 | 200 µg/ml | $364.00 | |||
CLK3 Antibody (D-10) Alexa Fluor® 546 | sc-365225 AF546 | 200 µg/ml | $364.00 | |||
CLK3 Antibody (D-10) Alexa Fluor® 594 | sc-365225 AF594 | 200 µg/ml | $364.00 | |||
CLK3 Antibody (D-10) Alexa Fluor® 647 | sc-365225 AF647 | 200 µg/ml | $364.00 | |||
CLK3 Antibody (D-10) Alexa Fluor® 680 | sc-365225 AF680 | 200 µg/ml | $364.00 | |||
CLK3 Antibody (D-10) Alexa Fluor® 790 | sc-365225 AF790 | 200 µg/ml | $364.00 | |||
CLK3 (D-10) Neutralizing Peptide | sc-365225 P | 100 µg/0.5 ml | $69.00 |