CLEC-4E Antibody (E-5): sc-390807
- CLEC-4E Antibody (E-5) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 44-89 mapping within an extracellular domain of CLEC-4E of human origin
- CLEC-4E Antibody (E-5) is recommended for detection of CLEC-4E of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CLEC-4E Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CLEC-4E Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with CLEC-4E Antibody (E-5) (see ordering information below).
CLEC-4E Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects human CLEC-4E by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CLEC-4E (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLEC-4E, also known as Mincle (Macrophage-inducible C-type lectin) or CLECSF9, is a 219 amino acid single-pass type II membrane protein that plays a crucial role in immune response. CLEC-4E is primarily expressed in monocytes and functions as a receptor that recognizes specific carbohydrate structures on pathogens, thereby facilitating immune cell activation and subsequent inflammatory response. CLEC-4E contains a C-type lectin domain, which is essential for binding to glycoproteins and other carbohydrate-containing molecules, highlighting CLEC-4E′s importance in pathogen recognition and immune signaling. Human CLEC-4E shares 67% sequence identity with mouse CLEC-4E, indicating a conserved function across species. CLEC-4E is located in a region associated with natural killer cell genes on human chromosome 12 and exists in multiple alternatively spliced isoforms, further underscoring CLEC-4E′s functional diversity in immune regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLEC-4E Antibody (E-5) References:
- Mincle Signaling Promotes Con A Hepatitis. | Greco, SH., et al. 2016. J Immunol. 197: 2816-27. PMID: 27559045
- Immune Recognition of Pathogen-Derived Glycolipids Through Mincle. | Miyake, Y. and Yamasaki, S. 2020. Adv Exp Med Biol. 1204: 31-56. PMID: 32152942
- Mincle/Syk Signalling Promotes Intestinal Mucosal Inflammation Through Induction of Macrophage Pyroptosis in Crohn's Disease. | Gong, W., et al. 2020. J Crohns Colitis. 14: 1734-1747. PMID: 32333776
- TH17 cells promote CNS inflammation by sensing danger signals via Mincle. | Zhang, Q., et al. 2022. Nat Commun. 13: 2406. PMID: 35504893
- A mechanism of self-lipid endocytosis mediated by the receptor Mincle. | Kostarnoy, AV., et al. 2022. Proc Natl Acad Sci U S A. 119: e2120489119. PMID: 35867828
- Research progress on Mincle as a multifunctional receptor. | Huang, X., et al. 2023. Int Immunopharmacol. 114: 109467. PMID: 36436471
- IL-4 and helminth infection downregulate MINCLE-dependent macrophage response to mycobacteria and Th17 adjuvanticity. | Schick, J., et al. 2023. Elife. 12: PMID: 36753434
- Mincle-GSDMD-mediated release of IL-1β small extracellular vesicles from hepatic macrophages in ethanol-induced liver injury. | Zhang, Q., et al. 2023. Hepatol Commun. 7: PMID: 37185170
- Structural basis for plastic glycolipid recognition of the C-type lectin Mincle. | Furukawa, A., et al. 2023. Structure. 31: 1077-1085.e5. PMID: 37348496
- SAP130 released by ferroptosis tubular epithelial cells promotes macrophage polarization via Mincle signaling in sepsis acute kidney injury. | Zhang, J., et al. 2024. Int Immunopharmacol. 129: 111564. PMID: 38320352
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLEC-4E Antibody (E-5) | sc-390807 | 200 µg/ml | $322.00 | |||
CLEC-4E Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-530269 | 200 µg Ab; 10 µg BP | $361.00 | |||
CLEC-4E Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-523690 | 200 µg Ab, 40 µg BP | $361.00 | |||
CLEC-4E Antibody (E-5): m-IgG2a BP-HRP Bundle | sc-547471 | 200 µg Ab; 10 µg BP | $361.00 | |||
CLEC-4E Antibody (E-5) AC | sc-390807 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CLEC-4E Antibody (E-5) HRP | sc-390807 HRP | 200 µg/ml | $322.00 | |||
CLEC-4E Antibody (E-5) FITC | sc-390807 FITC | 200 µg/ml | $336.00 | |||
CLEC-4E Antibody (E-5) PE | sc-390807 PE | 200 µg/ml | $349.00 | |||
CLEC-4E Antibody (E-5) Alexa Fluor® 488 | sc-390807 AF488 | 200 µg/ml | $364.00 | |||
CLEC-4E Antibody (E-5) Alexa Fluor® 546 | sc-390807 AF546 | 200 µg/ml | $364.00 | |||
CLEC-4E Antibody (E-5) Alexa Fluor® 594 | sc-390807 AF594 | 200 µg/ml | $364.00 | |||
CLEC-4E Antibody (E-5) Alexa Fluor® 647 | sc-390807 AF647 | 200 µg/ml | $364.00 | |||
CLEC-4E Antibody (E-5) Alexa Fluor® 680 | sc-390807 AF680 | 200 µg/ml | $364.00 | |||
CLEC-4E Antibody (E-5) Alexa Fluor® 790 | sc-390807 AF790 | 200 µg/ml | $364.00 |