CLC-2 Antibody (D-6): sc-377284

CLC-2 Antibody (D-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects CLC-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLC-2 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLC-2 protein is a member of the voltage-dependent chloride channel family, which plays a crucial role in regulating cellular trafficking of chloride ions, essential for maintaining cellular homeostasis and excitability in muscle and nerve cells. CLC-2 is predominantly expressed in epithelial tissues of several organs, including the lungs, where CLC-2 contributes to regulation of fluid transport and ion balance. This function is particularly significant in cystic fibrosis, as alterations in chloride ion transport can lead to accumulation of thick mucus in airways, making CLC-2 a potential therapeutic target for this condition. Expression patterns of other CLC family members, such as CLC-1 in skeletal muscle and CLC-3 in neuronal tissue, highlight diverse roles these channels play in various physiological processes. Understanding CLC-2 function and regulation is vital for elucidating involvement in both normal physiology and disease states.

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CLC-2 Antibody (D-6) References:

1. Expression of the chloride channel ClC-2 in the murine small intestine epithelium.  |  Gyömörey, K., et al. 2000. Am J Physiol Cell Physiol. 279: C1787-94. PMID: 11078693
2. The skeletal muscle chloride channel in dominant and recessive human myotonia.  |  Koch, MC., et al. 1992. Science. 257: 797-800. PMID: 1379744
3. ClC-2 channels regulate neuronal excitability, not intracellular chloride levels.  |  Ratté, S. and Prescott, SA. 2011. J Neurosci. 31: 15838-43. PMID: 22049427
4. ClC-2-like Chloride Current Alterations in a Cell Model of Spinal and Bulbar Muscular Atrophy, a Polyglutamine Disease.  |  Martínez-Rojas, VA., et al. 2021. J Mol Neurosci. 71: 662-674. PMID: 32856205
5. CryoEM structures of the human CLC-2 voltage gated chloride channel reveal a ball and chain gating mechanism.  |  Xu, M., et al. 2023. bioRxiv.. PMID: 37645939
6. Characterization of a human and murine gene (CLCN3) sharing similarities to voltage-gated chloride channels and to a yeast integral membrane protein.  |  Borsani, G., et al. 1995. Genomics. 27: 131-41. PMID: 7665160
7. Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues.  |  Cid, LP., et al. 1995. Hum Mol Genet. 4: 407-13. PMID: 7795595
8. A gene from the Xp22.3 region shares homology with voltage-gated chloride channels.  |  van Slegtenhorst, MA., et al. 1994. Hum Mol Genet. 3: 547-52. PMID: 8069296
9. Dent's disease, a renal Fanconi syndrome with nephrocalcinosis and kidney stones, is associated with a microdeletion involving DXS255 and maps to Xp11.22.  |  Pook, MA., et al. 1993. Hum Mol Genet. 2: 2129-34. PMID: 8111383
10. ClC-6 and ClC-7 are two novel broadly expressed members of the CLC chloride channel family.  |  Brandt, S. and Jentsch, TJ. 1995. FEBS Lett. 377: 15-20. PMID: 8543009

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CLC-2 Antibody (D-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CLC-2 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CLC-2 Antibody (D-6) Specifications

Alternate Product Name: CLC-2

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 859-897

Epitope Target: CLC-2

Epitope Species: human

Product Reactivity/Detection: CLC-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CLC-2 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin