Clathrin Heavy Chain/CLTC Antibody (A-8): sc-271178

Clathrin HC Antibody (A-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Clathrin HC of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Clathrin HC antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Clathrin, a crucial cytosolic coat protein, plays a significant role in receptor-mediated endocytosis, facilitating the engulfing of receptor proteins by clathrin-coated vesicles. Clathrin′s structure consists of three heavy chains and three light chains that non-covalently associate to form a distinctive triskelion shape. This triskelion structure is essential for Clathrin′s function, as the proximal segment of the heavy chain contains a binding site for light chains, critical for the assembly and stability of the clathrin coat. Proper formation of this coat enables efficient internalization of membrane proteins and lipids, influencing various cellular processes, including nutrient uptake and signal transduction.

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Clathrin Heavy Chain/CLTC Antibody (A-8) References:

1. Tyrosine phosphorylation of clathrin heavy chain under oxidative stress.  |  Ihara, Y., et al. 2002. Biochem Biophys Res Commun. 297: 353-60. PMID: 12237126
2. Effect of clathrin heavy chain- and alpha-adaptin-specific small inhibitory RNAs on endocytic accessory proteins and receptor trafficking in HeLa cells.  |  Hinrichsen, L., et al. 2003. J Biol Chem. 278: 45160-70. PMID: 12960147
3. In vitro, insulin receptor catalyses phosphorylation of clathrin heavy chain and a plasma membrane 180,000 molecular weight protein.  |  Goren, HJ., et al. 1991. Cell Signal. 3: 523-36. PMID: 1786203
4. Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake.  |  Kasprowicz, J., et al. 2008. J Cell Biol. 182: 1007-16. PMID: 18762582
5. Characterization of a temperature-sensitive vertebrate clathrin heavy chain mutant as a tool to study clathrin-dependent events in vivo.  |  Neumann-Staubitz, P., et al. 2010. PLoS One. 5: e12017. PMID: 20700507
6. Endocytosis-independent function of clathrin heavy chain in the control of basal NF-κB activation.  |  Kim, ML., et al. 2011. PLoS One. 6: e17158. PMID: 21364927
7. A novel disorder reveals clathrin heavy chain-22 is essential for human pain and touch development.  |  Nahorski, MS., et al. 2015. Brain. 138: 2147-60. PMID: 26068709
8. MiR-199a-5p suppresses tumorigenesis by targeting clathrin heavy chain in hepatocellular carcinoma.  |  Huang, GH., et al. 2017. Cell Biochem Funct. 35: 98-104. PMID: 28261837
9. Evidence for the interaction of alpha-actinin and calmodulin with the clathrin heavy chain.  |  Merisko, EM. 1985. Eur J Cell Biol. 39: 167-72. PMID: 2867905
10. Interaction between autophagic vesicles and the Coxiella-containing vacuole requires CLTC (clathrin heavy chain).  |  Latomanski, EA. and Newton, HJ. 2018. Autophagy. 14: 1710-1725. PMID: 29973118

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Clathrin Heavy Chain/CLTC Antibody (A-8) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Clathrin Heavy Chain/CLTC Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Clathrin Heavy Chain/CLTC Antibody (A-8) Specifications

Alternate Product Name: Clathrin

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1637-1674

Epitope Target: Clathrin HC

Epitope Species: human

Product Reactivity/Detection: Clathrin HC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Clathrin Heavy Chain/CLTC Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Clathrin Heavy Chain/CLTC antibody is Clathrin Heavy Chain/CLTC Antibody (TD.1): sc-12734.