CLASP2 Antibody (F-3): sc-376496

CLASP2 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLASP2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CLASP2 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLASP2, also known as cytoplasmic linker associated protein 2, KIAA0627, or hOrbit2, is a 1,294 amino acid protein characterized by five HEAT repeats and is primarily localized in the cytoplasm, cytoskeleton, kinetochore, and Golgi apparatus. CLASP2 expression is particularly prominent in brain tissue, where CLASP2 functions as a microtubule plus-end tracking protein, crucial for regulating dynamic microtubule stability and ensuring proper polarization of cytoplasmic microtubule arrays in migrating cells. CLASP2 interacts with several key proteins, including EB1, EB3, ELKS, and CLIP-115, which not only stabilizes microtubules but also plays a vital role in maintaining kinetochore stability and ensuring accurate chromosomal alignment during cell division. CLASP2 undergoes phosphorylation by glycogen synthase kinase 3 beta, a modification that reduces microtubule-binding capacity. Two isoforms of CLASP2, designated beta and gamma, arise from alternative splicing, further diversifying functional roles in cellular processes.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CLASP2 Antibody (F-3) References:

1. Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts.  |  Akhmanova, A., et al. 2001. Cell. 104: 923-35. PMID: 11290329
2. The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance.  |  Lee, H., et al. 2004. Neuron. 42: 913-26. PMID: 15207236
3. CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex.  |  Mimori-Kiyosue, Y., et al. 2005. J Cell Biol. 168: 141-53. PMID: 15631994
4. CLIPs and CLASPs and cellular dynamics.  |  Galjart, N. 2005. Nat Rev Mol Cell Biol. 6: 487-98. PMID: 15928712
5. Spatial regulation of CLASP affinity for microtubules by Rac1 and GSK3beta in migrating epithelial cells.  |  Wittmann, T. and Waterman-Storer, CM. 2005. J Cell Biol. 169: 929-39. PMID: 15955847
6. CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta.  |  Lansbergen, G., et al. 2006. Dev Cell. 11: 21-32. PMID: 16824950
7. Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment.  |  Mimori-Kiyosue, Y., et al. 2006. Genes Cells. 11: 845-57. PMID: 16866869
8. Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function.  |  Pereira, AL., et al. 2006. Mol Biol Cell. 17: 4526-42. PMID: 16914514

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CLASP2 Antibody (F-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CLASP2 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CLASP2 Antibody (F-3) Specifications

Alternate Product Name: CLASP2

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-60

Epitope Target: CLASP2

Epitope Species: human

Product Reactivity/Detection: CLASP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CLASP2 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin