CLASP2 Antibody (F-3): sc-376496
- CLASP2 Antibody (F-3) is a mouse monoclonal IgG1 κ CLASP2 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 21-60 mapping near the N-terminus of CLASP2 of human origin
- CLASP2 Antibody (F-3) is recommended for detection of CLASP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CLASP2 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CLASP2 Antibody (F-3) for WB and IHC(P) applications. These reagents are now offered in bundles with CLASP2 Antibody (F-3) (see ordering information below).
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CLASP2 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLASP2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CLASP2 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLASP2, also known as cytoplasmic linker associated protein 2, KIAA0627, or hOrbit2, is a 1,294 amino acid protein characterized by five HEAT repeats and is primarily localized in the cytoplasm, cytoskeleton, kinetochore, and Golgi apparatus. CLASP2 expression is particularly prominent in brain tissue, where CLASP2 functions as a microtubule plus-end tracking protein, crucial for regulating dynamic microtubule stability and ensuring proper polarization of cytoplasmic microtubule arrays in migrating cells. CLASP2 interacts with several key proteins, including EB1, EB3, ELKS, and CLIP-115, which not only stabilizes microtubules but also plays a vital role in maintaining kinetochore stability and ensuring accurate chromosomal alignment during cell division. CLASP2 undergoes phosphorylation by glycogen synthase kinase 3 beta, a modification that reduces microtubule-binding capacity. Two isoforms of CLASP2, designated beta and gamma, arise from alternative splicing, further diversifying functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLASP2 Antibody (F-3) References:
- Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts. | Akhmanova, A., et al. 2001. Cell. 104: 923-35. PMID: 11290329
- The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance. | Lee, H., et al. 2004. Neuron. 42: 913-26. PMID: 15207236
- CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. | Mimori-Kiyosue, Y., et al. 2005. J Cell Biol. 168: 141-53. PMID: 15631994
- CLIPs and CLASPs and cellular dynamics. | Galjart, N. 2005. Nat Rev Mol Cell Biol. 6: 487-98. PMID: 15928712
- Spatial regulation of CLASP affinity for microtubules by Rac1 and GSK3beta in migrating epithelial cells. | Wittmann, T. and Waterman-Storer, CM. 2005. J Cell Biol. 169: 929-39. PMID: 15955847
- CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta. | Lansbergen, G., et al. 2006. Dev Cell. 11: 21-32. PMID: 16824950
- Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment. | Mimori-Kiyosue, Y., et al. 2006. Genes Cells. 11: 845-57. PMID: 16866869
- Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function. | Pereira, AL., et al. 2006. Mol Biol Cell. 17: 4526-42. PMID: 16914514
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLASP2 Antibody (F-3) | sc-376496 | 200 µg/ml | $316.00 | |||
CLASP2 Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-529882 | 200 µg Ab; 10 µg BP | $354.00 | |||
CLASP2 Antibody (F-3): m-IgGκ BP-HRP Bundle | sc-522975 | 200 µg Ab, 40 µg BP | $354.00 | |||
CLASP2 Antibody (F-3): m-IgG1 BP-HRP Bundle | sc-543741 | 200 µg Ab; 20 µg BP | $354.00 | |||
CLASP2 Antibody (F-3) AC | sc-376496 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CLASP2 Antibody (F-3) HRP | sc-376496 HRP | 200 µg/ml | $316.00 | |||
CLASP2 Antibody (F-3) FITC | sc-376496 FITC | 200 µg/ml | $330.00 | |||
CLASP2 Antibody (F-3) PE | sc-376496 PE | 200 µg/ml | $343.00 | |||
CLASP2 Antibody (F-3) Alexa Fluor® 488 | sc-376496 AF488 | 200 µg/ml | $357.00 | |||
CLASP2 Antibody (F-3) Alexa Fluor® 546 | sc-376496 AF546 | 200 µg/ml | $357.00 | |||
CLASP2 Antibody (F-3) Alexa Fluor® 594 | sc-376496 AF594 | 200 µg/ml | $357.00 | |||
CLASP2 Antibody (F-3) Alexa Fluor® 647 | sc-376496 AF647 | 200 µg/ml | $357.00 | |||
CLASP2 Antibody (F-3) Alexa Fluor® 680 | sc-376496 AF680 | 200 µg/ml | $357.00 | |||
CLASP2 Antibody (F-3) Alexa Fluor® 790 | sc-376496 AF790 | 200 µg/ml | $357.00 |