CIZ1 Antibody (A-6): sc-393021

CIZ1 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects CIZ1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CIZ1 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CIZ1, or Cip1-interacting zinc finger protein, is an 898 amino acid nuclear protein encoded by the human gene CIZ1, belonging to the matrin 3 family and characterized by a single matrin-type zinc finger. CIZ1 contains two distinct functional domains: an N-terminal replication domain that is essential for interaction with replication machinery and a C-terminal nuclear matrix anchor that facilitates localization within the nucleus. The spatial distribution of CIZ1 is influenced by alternative splicing, particularly the exclusion of exon 4, which can affect isoform expression patterns and subsequently modulate DNA replication processes. Understanding the dynamics of CIZ1 and its interactions is vital for elucidating CIZ1′s role in cell cycle regulation and potential implications in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CIZ1 Antibody (A-6) References:

1. Ciz1, Cip1 interacting zinc finger protein 1 binds the consensus DNA sequence ARYSR(0-2)YYAC.  |  Warder, DE. and Keherly, MJ. 2003. J Biomed Sci. 10: 406-17. PMID: 12824700
2. Selective interactions of human kin17 and RPA proteins with chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner.  |  Miccoli, L., et al. 2003. Nucleic Acids Res. 31: 4162-75. PMID: 12853634
3. Initiation of DNA replication at a nuclear matrix-attached chromatin fraction.  |  Radichev, I., et al. 2005. J Cell Physiol. 203: 71-7. PMID: 15493011
4. Ciz1 promotes mammalian DNA replication.  |  Coverley, D., et al. 2005. J Cell Sci. 118: 101-12. PMID: 15585571
5. Nuclear matrix support of DNA replication.  |  Anachkova, B., et al. 2005. J Cell Biochem. 96: 951-61. PMID: 16167334
6. C-terminal domains deliver the DNA replication factor Ciz1 to the nuclear matrix.  |  Ainscough, JF., et al. 2007. J Cell Sci. 120: 115-24. PMID: 17182902
7. Cancer-associated missplicing of exon 4 influences the subnuclear distribution of the DNA replication factor CIZ1.  |  Rahman, F., et al. 2007. Hum Mutat. 28: 993-1004. PMID: 17508423
8. Ciz1, a p21 cip1/Waf1-interacting zinc finger protein and DNA replication factor, is a novel molecular partner for human enhancer of rudimentary homolog.  |  Lukasik, A., et al. 2008. FEBS J. 275: 332-40. PMID: 18081865

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CIZ1 Antibody (A-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CIZ1 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CIZ1 Antibody (A-6) Specifications

Alternate Product Name: CIZ1

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 521-740

Epitope Target: CIZ1

Epitope Species: human

Product Reactivity/Detection: CIZ1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CIZ1 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin