CIS Antibody (D-2): sc-166363

CIS Antibody (D-2) is a mouse monoclonal IgG1 antibody that detects CIS in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CIS antibody (D-2) specifically recognizes the cytokine-inducible SH2-containing protein, which plays a crucial role in mediating cytokine signaling pathways. CIS, consisting of 267 amino acids, features a single 96 amino acid SH2 domain that facilitates interaction with tyrosine-phosphorylated receptors, such as the IL-3 and Epo receptors. CIS association with these receptors is vital for the regulation of cellular responses to cytokines, influencing processes such as cell proliferation and differentiation. By modulating these signaling pathways, CIS serves as a unique regulator of cytokine signaling, making CIS an important target for research in immunology and cell biology. CIS (D-2) antibody′s versatility in various applications allows researchers to explore the intricate mechanisms of cytokine signaling and its implications in health and disease.

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CIS Antibody (D-2) References:

1. Cytokine-induced Src homology 2 protein (CIS) promotes T cell receptor-mediated proliferation and prolongs survival of activated T cells.  |  Li, S., et al. 2000. J Exp Med. 191: 985-94. PMID: 10727460
2. A complex interaction pattern of CIS and SOCS2 with the leptin receptor.  |  Lavens, D., et al. 2006. J Cell Sci. 119: 2214-24. PMID: 16684815
3. The C-terminus of CIS defines its interaction pattern.  |  Lavens, D., et al. 2007. Biochem J. 401: 257-67. PMID: 16961462
4. Expression of suppressor of cytokine signaling 3 (SOCS3) and cytokine-inducible Src homology 2-containing protein (CIS) induced in Burkholderia pseudomallei--infected mouse macrophages requires bacterial internalization.  |  Ekchariyawat, P., et al. 2007. Microb Pathog. 42: 104-10. PMID: 17240114
5. Mammalian protein-protein interaction trap (MAPPIT) analysis of STAT5, CIS, and SOCS2 interactions with the growth hormone receptor.  |  Uyttendaele, I., et al. 2007. Mol Endocrinol. 21: 2821-31. PMID: 17666591
6. Spontaneous resolution of acute gouty arthritis is associated with rapid induction of the anti-inflammatory factors TGFβ1, IL-10 and soluble TNF receptors and the intracellular cytokine negative regulators CIS and SOCS3.  |  Chen, YH., et al. 2011. Ann Rheum Dis. 70: 1655-63. PMID: 21613312
7. HIV-1 tat protein recruits CIS to the cytoplasmic tail of CD127 to induce receptor ubiquitination and proteasomal degradation.  |  Sugden, S., et al. 2016. Virology. 498: 192-200. PMID: 27596538
8. Phosphorylated CIS suppresses the Epo or JAK2 V617F mutant-triggered cell proliferation through binding to EpoR.  |  Funakoshi-Tago, M., et al. 2017. Cell Signal. 31: 41-57. PMID: 28038963
9. Treg expression of CIS suppresses allergic airway inflammation through antagonizing an autonomous TH2 program.  |  Zheng, H., et al. 2020. Mucosal Immunol. 13: 293-302. PMID: 31780776
10. NK Cell Priming From Endogenous Homeostatic Signals Is Modulated by CIS.  |  Delconte, RB., et al. 2020. Front Immunol. 11: 75. PMID: 32082327

The preferred CIS antibody is CIS Antibody (D-8): sc-74581.