CIP29 Antibody (F-4): sc-514567

CIP29 Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects CIP29 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CIP29 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CIP29, also known as Cytokine-induced protein of 29 kDa, SARNP (SAP domain-containing ribonucleoprotein), and HCC1 (Nuclear protein Hcc-1), is a 210 amino acid nuclear protein that plays a crucial role in cellular processes, particularly in cell cycle progression and apoptosis, as expression is upregulated by erythropoietin. CIP29 is notable for binding both single-stranded and double-stranded DNA, exhibiting a higher affinity for single-stranded DNA, which is essential for function as a transcription factor. CIP29 is expressed at low levels in various tissues, including pancreas, spleen, testis, liver, kidney, heart, and thymus, but is found at elevated levels in pancreatic adenocarcinoma and hepatocellular carcinoma, highlighting potential as a biomarker for these malignancies. Additionally, a translocation t(11;12)(q23;q13) in acute myelomonocytic leukemia results in the fusion of the coding region of CIP29 gene with exon 9 of the MLL gene, leading to the production of a hybrid protein that retains the N-terminal SAP domain and nuclear localization signals of CIP29, along with the AT hooks and DNA methyltransferase homology region from MLL, further emphasizing importance in cancer biology.

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CIP29 Antibody (F-4) References:

1. An integrated approach in the discovery and characterization of a novel nuclear protein over-expressed in liver and pancreatic tumors.  |  Choong, ML., et al. 2001. FEBS Lett. 496: 109-16. PMID: 11356193
2. Cloning and characterization of a proliferation-associated cytokine-inducible protein, CIP29.  |  Fukuda, S., et al. 2002. Biochem Biophys Res Commun. 292: 593-600. PMID: 11922608
3. A novel partner gene CIP29 containing a SAP domain with MLL identified in infantile myelomonocytic leukemia.  |  Hashii, Y., et al. 2004. Leukemia. 18: 1546-8. PMID: 15284855
4. Hcc-1 is a novel component of the nuclear matrix with growth inhibitory function.  |  Leaw, CL., et al. 2004. Cell Mol Life Sci. 61: 2264-73. PMID: 15338056
5. Growth inhibitory effect of Hcc-1/CIP29 is associated with induction of apoptosis, not just with G2/M arrest.  |  Fukuda, S. and Pelus, LM. 2005. Cell Mol Life Sci. 62: 1526-7. PMID: 15924260
6. Intracellular characterization of DDX39, a novel growth-associated RNA helicase.  |  Sugiura, T., et al. 2007. Exp Cell Res. 313: 782-90. PMID: 17196963

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CIP29 Antibody (F-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CIP29 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CIP29 Antibody (F-4) Specifications

Alternate Product Name: CIP29

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 77-210

Epitope Target: CIP29

Epitope Species: human

Product Reactivity/Detection: CIP29

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CIP29 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin