Chx10 Antibody (D-11): sc-374151

Chx10 Antibody (D-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Chx10 protein from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Chx10 antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Chx10, also known as ceh-10 homeodomain containing homolog, RET1, and HOX10, plays a crucial role in mammalian eye development, particularly influencing retinal progenitor proliferation and bipolar cell specification. Chx10 is primarily located in early retinal neuroepithelium and specifically expressed in bipolar cells, maintaining low levels in mature retina. Chx10 expression in the inner nuclear layer of mature retina highlights its importance in neuroretina formation and inner nuclear layer development and maintenance. Chx10 shows high expression in uncommitted retinal progenitor cells, indicating an essential function in early retinal development. Chx10′s ability to regulate key developmental processes makes this protein a vital target for studies related to retinal biology and associated disorders.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Chx10 Antibody (D-11) References:

1. Human microphthalmia associated with mutations in the retinal homeobox gene CHX10.  |  Ferda Percin, E., et al. 2000. Nat Genet. 25: 397-401. PMID: 10932181
2. Delayed expression of the Crx gene and photoreceptor development in the Chx10-deficient retina.  |  Rutherford, AD., et al. 2004. Invest Ophthalmol Vis Sci. 45: 375-84. PMID: 14744875
3. Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter.  |  Rowan, S. and Cepko, CL. 2004. Dev Biol. 271: 388-402. PMID: 15223342
4. Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10.  |  Rowan, S., et al. 2004. Development. 131: 5139-52. PMID: 15459106
5. Transcriptional activity of the paired-like homeodomain proteins CHX10 and VSX1.  |  Dorval, KM., et al. 2005. J Biol Chem. 280: 10100-8. PMID: 15647262
6. A POU factor binding site upstream of the Chx10 homeobox gene is required for Chx10 expression in subsets of retinal progenitor cells and bipolar cells.  |  Rowan, S. and Cepko, CL. 2005. Dev Biol. 281: 240-55. PMID: 15893976
7. Negative regulation of Vsx1 by its paralog Chx10/Vsx2 is conserved in the vertebrate retina.  |  Clark, AM., et al. 2008. Brain Res. 1192: 99-113. PMID: 17919464
8. Chx10 Consolidates V2a Interneuron Identity through Two Distinct Gene Repression Modes.  |  Clovis, YM., et al. 2016. Cell Rep. 16: 1642-1652. PMID: 27477290
9. Vsx1 and Chx10 paralogs sequentially secure V2 interneuron identity during spinal cord development.  |  Debrulle, S., et al. 2020. Cell Mol Life Sci. 77: 4117-4131. PMID: 31822965
10. mTORC1 Activation in Chx10-Specific Tsc1 Knockout Mice Accelerates Retina Aging and Degeneration.  |  Rao, YQ., et al. 2021. Oxid Med Cell Longev. 2021: 6715758. PMID: 34777691

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Chx10 Antibody (D-11) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-Chx10 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Chx10 Antibody (D-11) Specifications

Alternate Product Name: Chx10

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 37-64

Epitope Target: Chx10

Epitope Species: human

Product Reactivity/Detection: Chx10

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for Chx10 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Chx10 antibody is Chx10 Antibody (E-12): sc-365519.