Chx10 Antibody (E-12): sc-365519

Chx10 Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Chx10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Chx10 antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Chx10, also known as Visual System Homeobox 2, plays a crucial role in mammalian eye development, particularly in retinal progenitor cell proliferation and bipolar cell specification. Chx10 expression is primarily localized in early retinal neuroepithelium and is restricted to bipolar cells, maintaining low levels in mature retina. Chx10 shows high expression in uncommitted retinal progenitor cells, indicating significance in early retinal development stages. Chx10 interaction with other key factors in retinal development pathway underscores importance in formation and maintenance of retinal inner nuclear layer. Precise regulation of Chx10 expression is vital for proper retinal architecture and function, making anti-Chx10 antibody (E-12) an essential tool for researchers studying retinal development and related disorders.

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Chx10 Antibody (E-12) References:

1. Expression of Chx10 and Chx10-1 in the developing chicken retina.  |  Chen, CM. and Cepko, CL. 2000. Mech Dev. 90: 293-7. PMID: 10640715
2. Human microphthalmia associated with mutations in the retinal homeobox gene CHX10.  |  Ferda Percin, E., et al. 2000. Nat Genet. 25: 397-401. PMID: 10932181
3. Chx10 repression of Mitf is required for the maintenance of mammalian neuroretinal identity.  |  Horsford, DJ., et al. 2005. Development. 132: 177-87. PMID: 15576400
4. CHX10 targets a subset of photoreceptor genes.  |  Dorval, KM., et al. 2006. J Biol Chem. 281: 744-51. PMID: 16236706
5. Absence of chx10 causes neural progenitors to persist in the adult retina.  |  Dhomen, NS., et al. 2006. Invest Ophthalmol Vis Sci. 47: 386-96. PMID: 16384989
6. Chx10 is required to block photoreceptor differentiation but is dispensable for progenitor proliferation in the postnatal retina.  |  Livne-Bar, I., et al. 2006. Proc Natl Acad Sci U S A. 103: 4988-93. PMID: 16547132
7. Intrinsic brainstem circuits comprised of Chx10-expressing neurons contribute to reticulospinal output in mice.  |  Chopek, JW., et al. 2021. J Neurophysiol. 126: 1978-1990. PMID: 34669520
8. Chx10+V2a interneurons in spinal motor regulation and spinal cord injury.  |  Li, WY., et al. 2023. Neural Regen Res. 18: 933-939. PMID: 36254971
9. Pedunculopontine Chx10+ neurons control global motor arrest in mice.  |  Goñi-Erro, H., et al. 2023. Nat Neurosci. 26: 1516-1528. PMID: 37501003
10. The C. elegans neuronally expressed homeobox gene ceh-10 is closely related to genes expressed in the vertebrate eye.  |  Svendsen, PC. and McGhee, JD. 1995. Development. 121: 1253-62. PMID: 7789259
11. Developmental expression of a novel murine homeobox gene (Chx10): evidence for roles in determination of the neuroretina and inner nuclear layer.  |  Liu, IS., et al. 1994. Neuron. 13: 377-93. PMID: 7914735
12. Ocular retardation mouse caused by Chx10 homeobox null allele: impaired retinal progenitor proliferation and bipolar cell differentiation.  |  Burmeister, M., et al. 1996. Nat Genet. 12: 376-84. PMID: 8630490

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Chx10 Antibody (E-12) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-Chx10 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Chx10 Antibody (E-12) Specifications

Alternate Product Name: VSX2

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 37-64

Epitope Target: Chx10

Epitope Species: human

Product Reactivity/Detection: Chx10

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for Chx10 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Chx10 antibody is Chx10 Antibody (D-11): sc-374151.