CHMP5 Antibody (F-7): sc-374338

CHMP5 Antibody (F-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects CHMP5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CHMP5 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CHMP5 is a vital component of the endosomal sorting complex required for transport (ESCRT) machinery, specifically involved in the formation of multivesicular bodies (MVBs). These structures play a crucial role in sorting and degrading cellular proteins, as well as regulating signal transduction pathways. CHMP5 interacts with LIP5, a protein essential for HIV-1 release, highlighting CHMP5′s importance in viral budding processes. The balance of CHMP5 and LIP5 levels is critical; depletion of LIP5 reduces HIV-1 budding, while depletion of CHMP5 increases viral release, indicating CHMP5′s role as a negative regulator of HIV-1 budding. CHMP5 is involved in late endosomal development, and CHMP5 loss can lead to enhanced signal transduction due to impaired lysosomal degradation. This multifaceted role underscores CHMP5′s significance in both cellular homeostasis and viral pathogenesis, making anti-CHMP5 antibody (F-7) an essential tool for researchers studying these processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CHMP5 Antibody (F-7) References:

1. The protein network of HIV budding.  |  von Schwedler, UK., et al. 2003. Cell. 114: 701-13. PMID: 14505570
2. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.  |  Ward, DM., et al. 2005. J Biol Chem. 280: 10548-55. PMID: 15644320
3. CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis.  |  Shim, JH., et al. 2006. J Cell Biol. 172: 1045-56. PMID: 16567502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CHMP5 Antibody (F-7) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CHMP5 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CHMP5 Antibody (F-7) Specifications

Alternate Product Name: CHMP5

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 130-219

Epitope Target: CHMP5

Epitope Species: human

Product Reactivity/Detection: CHMP5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CHMP5 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin