cHMGCS Antibody (H-9): sc-373681

cHMGCS Antibody (H-9) is a mouse monoclonal IgG1 antibody that detects cHMGCS in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). cHMGCS, or cytoplasmic HMG-CoA synthase, plays a crucial role in the mevalonate pathway, which is essential for the biosynthesis of isoprenoids, including cholesterol and other vital biomolecules. cHMGCS catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA to form HMG-CoA, which is subsequently converted into mevalonate by HMG-CoA reductase, marking the beginning of isoprenoid biosynthesis. cHMGCS functions extend beyond cholesterol production, contributing to the synthesis of ubiquinone, dolichol, and farnesyl groups, all critical for cellular function and membrane integrity. cHMGCS is predominantly expressed in the liver and kidney, and expression is tightly regulated by nutritional states, with fasting and elevated levels of cAMP and fatty acids enhancing transcription, while feeding and insulin exert a repressive effect. The regulatory mechanisms governing cHMGCS expression involve a complex interplay of transcription factors, including PPAR, RXR, COUP-TF, and HNF-4, which bind to specific elements within the cHMGCS promoter, highlighting the enzyme′s significance in metabolic regulation and potential implications in metabolic disorders.

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cHMGCS Antibody (H-9) References:

1. HMGCS2 promotes autophagic degradation of the amyloid-β precursor protein through ketone body-mediated mechanisms.  |  Hu, LT., et al. 2017. Biochem Biophys Res Commun. 486: 492-498. PMID: 28320515
2. miR-107-mediated decrease of HMGCS2 indicates poor outcomes and promotes cell migration in hepatocellular carcinoma.  |  Su, SG., et al. 2017. Int J Biochem Cell Biol. 91: 53-59. PMID: 28867541
3. HMGCS2 functions as a tumor suppressor and has a prognostic impact in prostate cancer.  |  Wan, S., et al. 2019. Pathol Res Pract. 215: 152464. PMID: 31176575
4. Regulation of Ketogenic Enzyme HMGCS2 by Wnt/β-catenin/PPARγ Pathway in Intestinal Cells.  |  Kim, JT., et al. 2019. Cells. 8: PMID: 31546785
5. HMGCS2 Mediates Ketone Production and Regulates the Proliferation and Metastasis of Hepatocellular Carcinoma.  |  Wang, YH., et al. 2019. Cancers (Basel). 11: PMID: 31779269
6. HMGCS2 in metabolic pathways was associated with overall survival in hepatocellular carcinoma: A LASSO-derived study.  |  Ding, R., et al. 2021. Sci Prog. 104: 368504211031749. PMID: 34260294
7. HMGCS2 Mediation of Ketone Levels Affects Sorafenib Treatment Efficacy in Liver Cancer Cells.  |  Suk, FM., et al. 2022. Molecules. 27: PMID: 36432116
8. Sodium butyrate activates HMGCS2 to promote ketone body production through SIRT5-mediated desuccinylation.  |  Xu, Y., et al. 2023. Front Med. 17: 339-351. PMID: 36602721
9. 1,25(OH)2D3 alleviates oxidative stress and inflammation through up-regulating HMGCS2 in DSS-induced colitis.  |  Wang, HQ., et al. 2023. Int Immunopharmacol. 125: 111131. PMID: 38149572
10. A novel lncRNA LOC101928222 promotes colorectal cancer angiogenesis by stabilizing HMGCS2 mRNA and increasing cholesterol synthesis.  |  Chang, L., et al. 2024. J Exp Clin Cancer Res. 43: 185. PMID: 38965575

The preferred cHMGCS antibody is cHMGCS Antibody (A-6): sc-166763.