Chk1 Antibody (G-4): sc-8408

Chk1 Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Chk1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Chk1 (G-4) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Chk1 plays a critical role in the regulation of the cell cycle, particularly at the G2 DNA damage checkpoint, where Chk1 acts as a key regulator of cyclin-dependent kinases (Cdks). By phosphorylating Cdc25C in response to DNA damage, Chk1 inhibits Cdc25C activity, effectively blocking the transition to mitosis and allowing for DNA repair. This function is vital for maintaining genomic stability and preventing the propagation of damaged DNA, which can lead to tumorigenesis. Additionally, Chk1 has been shown to phosphorylate Wee1, further illustrating Chk1′s role in cell cycle regulation. The ability of Chk1 to modulate these critical processes underscores Chk1′s importance in cellular responses to DNA damage and highlights Chk1′s potential as a target for therapeutic intervention in cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Chk1 Antibody (G-4) References:

1. Cross-talk between Chk1 and Chk2 in double-mutant thymocytes.  |  Zaugg, K., et al. 2007. Proc Natl Acad Sci U S A. 104: 3805-10. PMID: 17360434
2. Exercising restraints: role of Chk1 in regulating the onset and progression of unperturbed mitosis in vertebrate cells.  |  Zachos, G. and Gillespie, DA. 2007. Cell Cycle. 6: 810-3. PMID: 17377502
3. HCLK2 is essential for the mammalian S-phase checkpoint and impacts on Chk1 stability.  |  Collis, SJ., et al. 2007. Nat Cell Biol. 9: 391-401. PMID: 17384638
4. cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
5. The ATM-Chk2 and ATR-Chk1 pathways in DNA damage signaling and cancer.  |  Smith, J., et al. 2010. Adv Cancer Res. 108: 73-112. PMID: 21034966
6. ATR/CHK1 inhibitors and cancer therapy.  |  Qiu, Z., et al. 2018. Radiother Oncol. 126: 450-464. PMID: 29054375
7. UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.  |  Sun, J., et al. 2020. J Exp Clin Cancer Res. 39: 222. PMID: 33087136
8. A new twist to the cell cycle.  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
9. Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation.  |  O'Connell, MJ., et al. 1997. EMBO J. 16: 545-54. PMID: 9034337
10. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.  |  Sanchez, Y., et al. 1997. Science. 277: 1497-501. PMID: 9278511
11. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.  |  Peng, CY., et al. 1997. Science. 277: 1501-5. PMID: 9278512
12. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.  |  Matsuoka, S., et al. 1998. Science. 282: 1893-7. PMID: 9836640

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Chk1 Antibody (G-4) has 841 citations in a variety of scientific publications.

Primary antibodies like Anti-Chk1 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Chk1 Antibody (G-4) Specifications

Alternate Product Name: CHEK1

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-476

Epitope Target: Chk1

Epitope Species: human

Product Reactivity/Detection: Chk1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 841 citations in various scientific publications

Available Conjugates/Forms for Chk1 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin