CHIP/STUB1 Antibody (C-10): sc-133083

CHIP Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that specifically binds to the C-terminus of the human CHIP protein, recognizing amino acids 73-303. CHIP, also known as STUB1 (STIP1 homology and U-box containing protein 1), is a cytoplasmic E3 ubiquitin ligase that plays a crucial role in protein quality control by mediating the ubiquitination and subsequent degradation of misfolded or damaged proteins. Located predominantly in the cytoplasm, CHIP interacts with molecular chaperones Hsp70 and Hsp90, facilitating the degradation of their client proteins and thus maintaining cellular protein homeostasis. The cytoplasmic localization of CHIP is essential for monitoring and regulating the quality of newly synthesized proteins, preventing the accumulation of aberrant proteins that could lead to cellular dysfunction or disease. CHIP also modulates signaling pathways by interacting with proteins such as Smad1/Smad4, inhibiting BMP signaling through ubiquitin-mediated degradation of Smad proteins. Additionally, CHIP regulates the stability of oncogenic proteins like ErbB2 by controlling their association with chaperones and promoting their degradation. Expression of CHIP is most abundant in striated muscle tissues, including heart and skeletal muscle, with lower levels observed in the pancreas and brain. Anti-CHIP antibody (C-10) reacts with CHIP from mouse, rat, and human origins and is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CHIP/STUB1 Antibody (C-10) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-CHIP/STUB1 Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CHIP/STUB1 Antibody (C-10) Specifications

Alternate Product Name: CHIP

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 73-303

Epitope Target: CHIP

Epitope Species: human

Product Reactivity/Detection: CHIP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for CHIP/STUB1 Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CHIP/STUB1 antibody is CHIP/STUB1 Antibody (G-2): sc-133066.