CHIP/STUB1 Antibody (G-2): sc-133066

CHIP Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that specifically binds to the human CHIP protein, recognizing an epitope corresponding to amino acids 73-303 at the C-terminus. CHIP, also known as STUB1 (STIP1 homology and U-box containing protein 1), is a cytoplasmic E3 ubiquitin ligase that plays a crucial role in protein quality control by mediating the ubiquitination and subsequent degradation of misfolded or damaged proteins. This function is vital for maintaining cellular homeostasis and preventing the accumulation of potentially toxic proteins that can lead to disease. CHIP interacts with molecular chaperones Hsp70 and Hsp90, facilitating the degradation of client proteins such as ErbB2 and CFTR, which is significant in regulating signaling pathways and stress responses. Additionally, CHIP can modulate signaling pathways by interacting with Smad1/Smad4 and inhibiting BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP is most abundantly expressed in striated muscle tissues, including heart and skeletal muscle, with lower levels observed in the pancreas and brain. Anti-CHIP antibody (G-2) reacts with CHIP from mouse, rat, and human origins and is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications.

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CHIP/STUB1 Antibody (G-2) References:

1. Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions.  |  Ballinger, CA., et al. 1999. Mol Cell Biol. 19: 4535-45. PMID: 10330192
2. CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation.  |  Jiang, J., et al. 2001. J Biol Chem. 276: 42938-44. PMID: 11557750
3. CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity.  |  Imai, Y., et al. 2002. Mol Cell. 10: 55-67. PMID: 12150907
4. Chaperone-dependent E3 ubiquitin ligase CHIP mediates a degradative pathway for c-ErbB2/Neu.  |  Xu, W., et al. 2002. Proc Natl Acad Sci U S A. 99: 12847-52. PMID: 12239347
5. Chaperone-dependent regulation of endothelial nitric-oxide synthase intracellular trafficking by the co-chaperone/ubiquitin ligase CHIP.  |  Jiang, J., et al. 2003. J Biol Chem. 278: 49332-41. PMID: 14507928
6. The cochaperone HspBP1 inhibits the CHIP ubiquitin ligase and stimulates the maturation of the cystic fibrosis transmembrane conductance regulator.  |  Alberti, S., et al. 2004. Mol Biol Cell. 15: 4003-10. PMID: 15215316
7. Intracellular localization of ornithine decarboxylase and its regulatory protein, antizyme-1.  |  Schipper, RG., et al. 2004. J Histochem Cytochem. 52: 1259-66. PMID: 15385572
8. A foldable CFTR{Delta}F508 biogenic intermediate accumulates upon inhibition of the Hsc70-CHIP E3 ubiquitin ligase.  |  Younger, JM., et al. 2004. J Cell Biol. 167: 1075-85. PMID: 15611333

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CHIP/STUB1 Antibody (G-2) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-CHIP/STUB1 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CHIP/STUB1 Antibody (G-2) Specifications

Alternate Product Name: CHIP

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 73-303

Epitope Target: CHIP

Epitope Species: human

Product Reactivity/Detection: CHIP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for CHIP/STUB1 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CHIP/STUB1 antibody is CHIP/STUB1 Antibody (C-10): sc-133083.