Chibby Antibody (G-4): sc-393327

Chibby Antibody (G-4) is a mouse monoclonal IgG1 antibody that detects Chibby in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Chibby, also known as Cytosolic leucine-rich protein or PIGEA-14, is a highly conserved protein consisting of 126 amino acids that plays a crucial role in inhibiting β-catenin-mediated transcriptional activation by competing with LEF-1 for binding to β-catenin. This function is particularly important as it helps regulate the Wnt signaling pathway, which is vital for various cellular processes, including cell proliferation and differentiation. Chibby is primarily localized in the nucleus and the Golgi apparatus within the trans-Golgi network, where Chibby acts as a homodimer. Interaction with the 14-3-3 protein leads to the sequestration of Chibby in the cytoplasm, forming a stable complex with β-catenin that facilitates the nuclear export of β-catenin, thereby modulating β-catenin activity. While Chibby is widely expressed, Chibby is found at elevated levels in tissues such as skeletal muscle, heart, placenta, and kidney. Notably, down-regulation of Chibby has been observed in thyroid and metastatic uterine tumors, indicating Chibby′s potential role as a tumor suppressor. Anti-Chibby antibody (G-4) is an essential tool for researchers investigating the intricate mechanisms of cell signaling and cancer biology.

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Chibby Antibody (G-4) References:

1. Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway.  |  Takemaru, K., et al. 2003. Nature. 422: 905-9. PMID: 12712206
2. Chibby, an antagonist of the Wnt/beta-catenin pathway, facilitates cardiomyocyte differentiation of murine embryonic stem cells.  |  Singh, AM., et al. 2007. Circulation. 115: 617-26. PMID: 17261658
3. Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity.  |  Li, FQ., et al. 2008. J Cell Biol. 181: 1141-54. PMID: 18573912
4. Chibby forms a homodimer through a heptad repeat of leucine residues in its C-terminal coiled-coil motif.  |  Mofunanya, A., et al. 2009. BMC Mol Biol. 10: 41. PMID: 19435523
5. Chibby interacts with NBPF1 and clusterin, two candidate tumor suppressors linked to neuroblastoma.  |  Vandepoele, K., et al. 2010. Exp Cell Res. 316: 1225-33. PMID: 20096688
6. The expression patterns and correlations of chibby, β-catenin, and DNA methyltransferase-1 and their clinicopathological significance in lung cancers.  |  Xu, HT., et al. 2011. APMIS. 119: 750-8. PMID: 21995627
7. Drosophila chibby is required for basal body formation and ciliogenesis but not for Wg signaling.  |  Enjolras, C., et al. 2012. J Cell Biol. 197: 313-25. PMID: 22508513
8. Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.  |  Burke, MC., et al. 2014. J Cell Biol. 207: 123-37. PMID: 25313408
9. Downregulation of nuclear and cytoplasmic Chibby is associated with advanced cervical cancer.  |  Yang, MC., et al. 2017. Oncol Lett. 14: 6632-6644. PMID: 29181101
10. Chibby suppresses aerobic glycolysis and proliferation of nasopharyngeal carcinoma via the Wnt/β-catenin-Lin28/let7-PDK1 cascade.  |  Cai, CF., et al. 2018. J Exp Clin Cancer Res. 37: 104. PMID: 29764469

The preferred Chibby antibody is Chibby Antibody (C-10): sc-393295.