cGKII Antibody (E-7): sc-390926
- cGKII Antibody (E-7) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1-120 mapping at the N-terminus of cGKII of human origin
- recommended for detection of cGKII of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for cGKII Antibody (E-7) for WB applications. These reagents are now offered in bundles with cGKII Antibody (E-7) (see ordering information below).
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cGKII Antibody (E-7) is a mouse monoclonal IgG2a antibody that detects cGKII in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). cGKII, or cGMP-dependent protein kinase type II, plays a crucial role as a major receptor for intracellular cyclic guanosine monophosphate (cGMP), mediating a wide array of physiological responses essential for maintaining cellular homeostasis. cGKII is predominantly expressed in the small intestine, colon, prostate, and human brain tissues, with its gene located on chromosome 4q21.1. Notably, cGKII contains a conserved leucine zipper motif at its amino-terminus, which is important for protein-protein interactions and stability. The myristoylation of the penultimate glycine residue in cGKII is particularly significant, as this modification directs cGKII to the membrane, enabling proper function in regulating intestinal ion transport. Since cGKII lacks hydrophobic transmembrane domains, membrane localization through myristoylation is vital for physiological role. This modification ensures effective regulation of ion transport processes, which are essential for nutrient absorption and electrolyte balance in the intestine.
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cGKII Antibody (E-7) References:
- Autophosphorylation of cGMP-dependent protein kinase type II. | Vaandrager, AB., et al. 2003. J Biol Chem. 278: 28651-8. PMID: 12764134
- A GluR1-cGKII interaction regulates AMPA receptor trafficking. | Serulle, Y., et al. 2007. Neuron. 56: 670-88. PMID: 18031684
- Cyclic GMP kinase II (cGKII) inhibits NHE3 by altering its trafficking and phosphorylating NHE3 at three required sites: identification of a multifunctional phosphorylation site. | Chen, T., et al. 2015. J Biol Chem. 290: 1952-65. PMID: 25480791
- Transepithelial Fluid and Salt Re-Absorption Regulated by cGK2 Signals. | Chang, J., et al. 2018. Int J Mol Sci. 19: PMID: 29547542
- Inhibition of Cgkii Suppresses Seizure Activity and Hippocampal Excitation by Regulating the Postsynaptic Delivery of Glua1. | Gu, J., et al. 2018. Cell Physiol Biochem. 46: 160-177. PMID: 29587280
- Expression of PKG2 in ovarian cancer and its effect on epidermal growth factor receptor. | Xu, H., et al. 2020. J BUON. 25: 729-735. PMID: 32521860
- Biallelic cGMP-dependent type II protein kinase gene (PRKG2) variants cause a novel acromesomelic dysplasia. | Díaz-González, F., et al. 2022. J Med Genet. 59: 28-38. PMID: 33106379
- Pharmic Activation of PKG2 Alleviates Diabetes-Induced Osteoblast Dysfunction by Suppressing PLCβ1-Ca2+-Mediated Endoplasmic Reticulum Stress. | Jia, T., et al. 2021. Oxid Med Cell Longev. 2021: 5552530. PMID: 34221234
- Natural history of clinical features in two brothers with acromesomelic dysplasia related to PRKG2. | Mollaoğlu, E., et al. 2023. Clin Genet. 103: 574-579. PMID: 36504352
- N-terminal myristoylation is required for membrane localization of cGMP-dependent protein kinase type II. | Vaandrager, AB., et al. 1996. J Biol Chem. 271: 7025-9. PMID: 8636133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cGKII Antibody (E-7) | sc-390926 | 200 µg/ml | $316.00 | |||
cGKII Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-540870 | 200 µg Ab; 10 µg BP | $354.00 | |||
cGKII Antibody (E-7): m-IgG2a BP-HRP Bundle | sc-546967 | 200 µg Ab; 10 µg BP | $354.00 |