CFTR Antibody (A-3): sc-376683

CFTR Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CFTR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CFTR Antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The cystic fibrosis transmembrane conductance regulator (CFTR) protein plays a crucial role in maintaining the balance of salt and water on epithelial surfaces, which is vital for proper respiratory and digestive function. Located primarily in the apical membrane of epithelial cells, anti-CFTR antibody (A-3) detects a protein that regulates chloride ion transport, and CFTR dysfunction leads to the accumulation of thick mucus in organs such as the lungs and pancreas, contributing to the pathophysiology of cystic fibrosis. Additionally, CFTR mutations are linked to congenital bilateral absence of vas deferens, a condition that affects male fertility. Understanding the structure and function of CFTR is essential for developing targeted therapies for cystic fibrosis and related disorders, making anti-CFTR antibody (A-3) an invaluable tool for researchers studying these critical pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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CFTR Antibody (A-3) References:

1. The cystic fibrosis transmembrane conductance regulator and its function in epithelial transport.  |  Kunzelmann, K. 1999. Rev Physiol Biochem Pharmacol. 137: 1-70. PMID: 10207304
2. CFTR directly mediates nucleotide-regulated glutathione flux.  |  Kogan, I., et al. 2003. EMBO J. 22: 1981-9. PMID: 12727866
3. The spectrum of cystic fibrosis mutations.  |  Tsui, LC. 1992. Trends Genet. 8: 392-8. PMID: 1279852
4. Aquaporins and CFTR in ocular epithelial fluid transport.  |  Levin, MH. and Verkman, AS. 2006. J Membr Biol. 210: 105-15. PMID: 16868675
5. Improved maturation of CFTR by an ER export signal.  |  Wendeler, MW., et al. 2007. FASEB J. 21: 2352-8. PMID: 17392477
6. Testing and reporting ACMG cystic fibrosis mutation panel results.  |  Lebo, RV. and Grody, WW. 2007. Genet Test. 11: 11-31. PMID: 17394390
7. Variable penetrance and expressivity of the splice altering 5T sequence in the cystic fibrosis gene.  |  Lebo, RV. and Grody, WW. 2007. Genet Test. 11: 32-44. PMID: 17394391
8. In vitro prediction of stop-codon suppression by intravenous gentamicin in patients with cystic fibrosis: a pilot study.  |  Sermet-Gaudelus, I., et al. 2007. BMC Med. 5: 5. PMID: 17394637
9. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.  |  Riordan, JR., et al. 1989. Science. 245: 1066-73. PMID: 2475911
10. PHLPP1 regulates CFTR activity and lumen expansion through AMPK.  |  Lobert, VH., et al. 2022. Development. 149: PMID: 35997536
11. CFTR and outward rectifying chloride channels are distinct proteins with a regulatory relationship.  |  Gabriel, SE., et al. 1993. Nature. 363: 263-8. PMID: 7683773
12. Cystic fibrosis-type mutational analysis in the ATP-binding cassette transporter signature of human P-glycoprotein MDR1.  |  Hoof, T., et al. 1994. J Biol Chem. 269: 20575-83. PMID: 7914197

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CFTR Antibody (A-3) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-CFTR Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CFTR Antibody (A-3) Specifications

Alternate Product Name: CFTR

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1419-1457

Epitope Target: CFTR

Epitope Species: human

Product Reactivity/Detection: CFTR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for CFTR Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin