ceruloplasmin Antibody (H-3): sc-365205

Ceruloplasmin Antibody (H-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects ceruloplasmin from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ceruloplasmin antibody (H-3) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ceruloplasmin (CP) is a blue plasma glycoprotein synthesized in hepatocytes, playing a crucial role in copper transport throughout the body. Known as ferroxidase, ceruloplasmin consists of three homologous domains resulting from intragenic triplication, existing in two splice variants, CP-1 and CP-2, which show differential expression in specific tissues. Ceruloplasmin is primarily expressed in human liver, macrophages, and lymphocytes, where ceruloplasmin binds copper, typically carrying six or seven cupric ions per molecule. Ceruloplasmin participates in iron oxidation, converting Fe(II) transferrin to Fe(III) transferrin, a process essential for iron metabolism. The structural integrity of ceruloplasmin is vital, as only the intact long form can effectively catalyze iron loading into ferritin, highlighting the importance of its three-dimensional structure in enzymatic function. Ceruloplasmin can induce low-density lipoprotein oxidation in vitro, a process requiring a single, chelatable copper atom. A glycosyl phosphatidylinositol (GPI)-anchored form of ceruloplasmin is expressed by Sertoli cells, suggesting potential significance in reproductive biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ceruloplasmin Antibody (H-3) References:

1. Rethinking the role of ceruloplasmin in brain iron metabolism.  |  Qian, ZM. and Ke, Y. 2001. Brain Res Brain Res Rev. 35: 287-94. PMID: 11423158
2. Ceruloplasmin immunoreactivity in neurodegenerative disorders.  |  Loeffler, DA., et al. 2001. Free Radic Res. 35: 111-8. PMID: 11697191
3. Relevance of copper and ceruloplasmin in psoriasis.  |  Rashmi, R., et al. 2010. Clin Chim Acta. 411: 1390-2. PMID: 20580696
4. Iron uptake and transfer from ceruloplasmin to transferrin.  |  Eid, C., et al. 2014. Biochim Biophys Acta. 1840: 1771-81. PMID: 24418516
5. Serum selenium and ceruloplasmin in nigerians with peripartum cardiomyopathy.  |  Karaye, KM., et al. 2015. Int J Mol Sci. 16: 7644-54. PMID: 25853263
6. Ceruloplasmin decreases respiratory burst reaction during pregnancy.  |  Varfolomeeva, EY., et al. 2016. Free Radic Res. 50: 909-19. PMID: 27266720
7. Correlations between ceruloplasmin, lactoferrin and myeloperoxidase in meconium.  |  Skarżyńska, E., et al. 2017. J Trace Elem Med Biol. 43: 58-62. PMID: 27903408
8. Hephaestin and ceruloplasmin facilitate iron metabolism in the mouse kidney.  |  Jiang, B., et al. 2016. Sci Rep. 6: 39470. PMID: 27991585
9. Autotolerant ceruloplasmin based biocathodes for implanted biological power sources.  |  Aleksejeva, O., et al. 2021. Bioelectrochemistry. 140: 107794. PMID: 33744681
10. Macular degeneration and elevated serum ceruloplasmin.  |  Newsome, DA., et al. 1986. Invest Ophthalmol Vis Sci. 27: 1675-80. PMID: 3793397
11. Protective role of ceruloplasmin in inflammation.  |  Denko, CW. 1979. Agents Actions. 9: 333-6. PMID: 517330
12. The role of ceruloplasmin in iron metabolism.  |  Roeser, HP., et al. 1970. J Clin Invest. 49: 2408-17. PMID: 5480864

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ceruloplasmin Antibody (H-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ceruloplasmin Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ceruloplasmin Antibody (H-3) Specifications

Alternate Product Name: ceruloplasmin

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 121-180

Epitope Target: ceruloplasmin

Epitope Species: human

Product Reactivity/Detection: ceruloplasmin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ceruloplasmin Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred antibody is Antibody (): .