CEP164 Antibody (E-9): sc-515403
- CEP164 Antibody (E-9) is a mouse monoclonal IgG2a κ CEP164 antibody, cited in 19 publications, provided at 200 µg/ml
- raised against amino acids 9-100 mapping at the N-terminus of CEP164 of human origin
- CEP164 Antibody (E-9) is recommended for detection of CEP164 of human origin by WB, IP, IF and ELISA
- Anti-CEP164 Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CEP164 Antibody (E-9) for WB applications. These reagents are now offered in bundles with CEP164 Antibody (E-9) (see ordering information below).
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CEP164 Antibody (E-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects CEP164 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CEP164 (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CEP164, also known as Centrosomal protein of 164 kDa, is a crucial component of the centrosome, which serves as the primary microtubule-organizing center in mammalian cells. CEP164 is essential for the proper assembly of the bipolar mitotic spindle, playing a significant role in centriole duplication and centrosome maturation. Disruptions in these processes can lead to spindle defects, resulting in aneuploidy and potentially contributing to cancer progression. CEP164 consists of 1,460 amino acids and maintains genomic stability during cell division, particularly at the G2/M checkpoint. CEP164 participates in the phosphorylation of key proteins such as Chk1, Chk2, Histone H2A, and RPA, which are critical for the DNA-damage-activated ATR/ATM signaling cascade. CEP164 localizes to the centrioles throughout mitosis, ensuring proper chromosomal segregation. Two isoforms of CEP164 are produced through alternative splicing, which may have distinct functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CEP164 Antibody (E-9) References:
- Centrosome composition and microtubule anchoring mechanisms. | Bornens, M. 2002. Curr Opin Cell Biol. 14: 25-34. PMID: 11792541
- Interaction of Cep135 with a p50 dynactin subunit in mammalian centrosomes. | Uetake, Y., et al. 2004. Cell Motil Cytoskeleton. 58: 53-66. PMID: 14983524
- Positioning centrosomes and spindle poles: looking at the periphery to find the centre. | Manneville, JB. and Etienne-Manneville, S. 2006. Biol Cell. 98: 557-65. PMID: 16907664
- Cep164, a novel centriole appendage protein required for primary cilium formation. | Graser, S., et al. 2007. J Cell Biol. 179: 321-30. PMID: 17954613
- Cep164 is a mediator protein required for the maintenance of genomic stability through modulation of MDC1, RPA, and CHK1. | Sivasubramaniam, S., et al. 2008. Genes Dev. 22: 587-600. PMID: 18283122
- UV-dependent interaction between Cep164 and XPA mediates localization of Cep164 at sites of DNA damage and UV sensitivity. | Pan, YR. and Lee, EY. 2009. Cell Cycle. 8: 655-64. PMID: 19197159
- CEP215 is involved in the dynein-dependent accumulation of pericentriolar matrix proteins for spindle pole formation. | Lee, S. and Rhee, K. 2010. Cell Cycle. 9: 774-83. PMID: 20139723
- Proteins required for centrosome clustering in cancer cells. | Leber, B., et al. 2010. Sci Transl Med. 2: 33ra38. PMID: 20505215
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEP164 Antibody (E-9) | sc-515403 | 200 µg/ml | $316.00 | |||
CEP164 Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-531353 | 200 µg Ab; 10 µg BP | $354.00 | |||
CEP164 Antibody (E-9): m-IgGκ BP-HRP Bundle | sc-525023 | 200 µg Ab, 40 µg BP | $354.00 | |||
CEP164 Antibody (E-9): m-IgG2a BP-HRP Bundle | sc-547770 | 200 µg Ab; 10 µg BP | $354.00 | |||
CEP164 Antibody (E-9) AC | sc-515403 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CEP164 Antibody (E-9) HRP | sc-515403 HRP | 200 µg/ml | $316.00 | |||
CEP164 Antibody (E-9) FITC | sc-515403 FITC | 200 µg/ml | $330.00 | |||
CEP164 Antibody (E-9) PE | sc-515403 PE | 200 µg/ml | $343.00 | |||
CEP164 Antibody (E-9) Alexa Fluor® 488 | sc-515403 AF488 | 200 µg/ml | $357.00 | |||
CEP164 Antibody (E-9) Alexa Fluor® 546 | sc-515403 AF546 | 200 µg/ml | $357.00 | |||
CEP164 Antibody (E-9) Alexa Fluor® 594 | sc-515403 AF594 | 200 µg/ml | $357.00 | |||
CEP164 Antibody (E-9) Alexa Fluor® 647 | sc-515403 AF647 | 200 µg/ml | $357.00 | |||
CEP164 Antibody (E-9) Alexa Fluor® 680 | sc-515403 AF680 | 200 µg/ml | $357.00 | |||
CEP164 Antibody (E-9) Alexa Fluor® 790 | sc-515403 AF790 | 200 µg/ml | $357.00 |