Centaurin α1 Antibody (G-4): sc-390498

Centaurin α1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Centaurin alpha 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Centaurin α1 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Centaurin alpha 1 protein plays a crucial role in cellular signaling and membrane trafficking, particularly through its function as a high-affinity phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding protein, which is predominantly enriched in the brain. Centaurin α1 is integral to the regulation of ADP-ribosylation factor 6 (ARF6), acting as a GTPase-activating protein that effectively switches off ARF6, thereby inhibiting its role in mediating β2-adrenoceptor internalization. Centaurin α1′s ability to negatively regulate ARF6 activity is vital for maintaining proper cellular functions, as this regulation ensures precise control of membrane traffic and cytoskeletal organization. Centaurin α1 is part of the Centaurin GTPase-activating protein family, which includes other important regulators such as ASAP1 and ACAP1, highlighting Centaurin α1′s significance in transducing phosphoinositide 3-kinase activation into coordinated control of ARF-dependent pathways.

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Centaurin α1 Antibody (G-4) References:

1. Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3,4,5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1.  |  Venkateswarlu, K., et al. 1999. Biochem J. 340 (Pt 2): 359-63. PMID: 10333475
2. Identification of centaurin-alpha2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle.  |  Whitley, P., et al. 2002. Eur J Cell Biol. 81: 222-30. PMID: 12018390
3. Cytohesins and centaurins control subcellular trafficking of macromolecular signaling complexes: regulation by phosphoinositides and ADP-ribosylation factors.  |  Hawadle, MA., et al. 2002. Biol Res. 35: 247-65. PMID: 12415743
4. Centaurin-alpha1 is an in vivo phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein for ARF6 that is involved in actin cytoskeleton organization.  |  Venkateswarlu, K., et al. 2004. J Biol Chem. 279: 6205-8. PMID: 14625293
5. The arf6 GAP centaurin alpha-1 is a neuronal actin-binding protein which also functions via GAP-independent activity to regulate the actin cytoskeleton.  |  Thacker, E., et al. 2004. Eur J Cell Biol. 83: 541-54. PMID: 15679100
6. Centaurin-alpha 1, an ADP-ribosylation factor 6 GTPase activating protein, inhibits beta 2-adrenoceptor internalization.  |  Lawrence, J., et al. 2005. Mol Pharmacol. 67: 1822-8. PMID: 15778454
7. Centaurin-alpha1 interacts directly with kinesin motor protein KIF13B.  |  Venkateswarlu, K., et al. 2005. J Cell Sci. 118: 2471-84. PMID: 15923660
8. Centaurin-alpha1 and KIF13B kinesin motor protein interaction in ARF6 signalling.  |  Kanamarlapudi, V. 2005. Biochem Soc Trans. 33: 1279-81. PMID: 16246098
9. Centaurin-alpha1 is a phosphatidylinositol 3-kinase-dependent activator of ERK1/2 mitogen-activated protein kinases.  |  Hayashi, H., et al. 2006. J Biol Chem. 281: 1332-7. PMID: 16287813
10. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation.  |  Moore, CD., et al. 2007. J Cell Sci. 120: 2683-93. PMID: 17635995
11. Functions of the neuron-specific protein ADAP1 (centaurin-α1) in neuronal differentiation and neurodegenerative diseases, with an overview of structural and biochemical properties of ADAP1.  |  Stricker, R. and Reiser, G. 2014. Biol Chem. 395: 1321-40. PMID: 24854535
12. ADAP1/Centaurin-α1 Negatively Regulates Dendritic Spine Function and Memory Formation in the Hippocampus.  |  Szatmari, EM., et al. 2021. eNeuro. 8: PMID: 33139322

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Centaurin α1 Antibody (G-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Centaurin α1 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Centaurin α1 Antibody (G-4) Specifications

Alternate Product Name: Centaurin α1

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 199-374

Epitope Target: Centaurin α1

Epitope Species: human

Product Reactivity/Detection: Centaurin α1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Centaurin α1 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin