CENP-E Antibody (mAb177): sc-47745

CENP-E Antibody (mAb177) is a mouse monoclonal IgG1 antibody that detects CENP-E in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). CENP-E is a crucial kinetochore motor protein that plays a significant role in chromosome segregation during mitosis, ensuring accurate distribution of replicated chromosomes to daughter cells. CENP-E function is vital for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy, a condition associated with various cancers and genetic disorders. During mitosis, CENP-E facilitates the binding of kinetochores to microtubules, which is essential for the proper alignment and movement of chromosomes. Additionally, CENP-E is expressed at high levels during meiosis I and II, where CENP-E localizes to the fibrous corona and outer plate of kinetochores on meiotic chromosomes, further underscoring CENP-E importance in cell division. Anti-CENP-E antibody (mAb177) is an invaluable tool for researchers studying the dynamics of cell division and the mechanisms underlying chromosomal abnormalities.

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CENP-E Antibody (mAb177) References:

1. Antitumor activity of an allosteric inhibitor of centromere-associated protein-E.  |  Wood, KW., et al. 2010. Proc Natl Acad Sci U S A. 107: 5839-44. PMID: 20167803
2. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase.  |  Yen, TJ., et al. 1991. EMBO J. 10: 1245-54. PMID: 2022189
3. CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites.  |  Xiao, T., et al. 2015. Cell Rep. 12: 1704-14. PMID: 26321640
4. Synthetic Studies on Centromere-Associated Protein-E (CENP-E) Inhibitors: 2. Application of Electrostatic Potential Map (EPM) and Structure-Based Modeling to Imidazo[1,2-a]pyridine Derivatives as Anti-Tumor Agents.  |  Hirayama, T., et al. 2015. J Med Chem. 58: 8036-53. PMID: 26372373
5. Motor activity of centromere-associated protein-E contributes to its localization at the center of the midbody to regulate cytokinetic abscission.  |  Ohashi, A., et al. 2016. Oncotarget. 7: 79964-79980. PMID: 27835888
6. A non-canonical function for Centromere-associated protein-E controls centrosome integrity and orientation of cell division.  |  Owa, M. and Dynlacht, B. 2021. Commun Biol. 4: 358. PMID: 33742057
7. Comprehensive pan-cancer analysis identifies centromere-associated protein E as a novel prognostic and immunological biomarker in human tumors.  |  Yuan, Y., et al. 2023. Biochim Biophys Acta Gen Subj. 1867: 130346. PMID: 36931353
8. Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System.  |  Xu, MF., et al. 2023. J Vis Exp.. PMID: 37427945
9. CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.  |  Lakshmi, RB., et al. 2024. EMBO Rep. 25: 1909-1935. PMID: 38424231
10. The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A.  |  Brown, KD., et al. 1996. J Cell Sci. 109 (Pt 5): 961-9. PMID: 8743943

The preferred CENP-E antibody is CENP-E Antibody (C-5): sc-376685.