CENP-B Antibody (2D-7): sc-32285
- CENP-B Antibody (2D-7) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against human CENP-B peptide
- recommended for detection of CENP-B of mouse, rat and human origin by WB, IP and IF
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CENP-B Antibody (2D-7) for WB applications. These reagents are now offered in bundles with CENP-B Antibody (2D-7) (see ordering information below).
CENP-B Antibody (2D-7) is a mouse monoclonal IgG1 antibody that detects CENP-B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). CENP-B plays a crucial role in the structure and function of centromeres, which are essential for accurate chromosome segregation during cell division. CENP-B is primarily located in the centromeric heterochromatin, where CENP-B facilitates the joining of DNA satellites, thereby maintaining sister chromatid cohesion. The unique coiled-coil structure of CENP-B not only aids in binding to centromeric a-DNA but also protects this DNA from degradation by nucleases, ensuring the integrity of centromere morphology. Additionally, CENP-B′s ability to target and stabilize centromeric regions is vital for the proper organization and function of kinetochores throughout the cell cycle, making CENP-B an important player in the maintenance of genomic stability. Anti-CENP-B antibody (2D-7) is an invaluable tool for researchers studying chromosome dynamics and the molecular mechanisms underlying cell division.
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CENP-B Antibody (2D-7) References:
- Circulating anticentromere CENP-A and CENP-B antibodies in patients with diffuse and limited systemic sclerosis, systemic lupus erythematosus, and rheumatoid arthritis. | Russo, K., et al. 2000. J Rheumatol. 27: 142-8. PMID: 10648030
- Clinical correlates of CENP-A and CENP-B antibodies in a large cohort of patients with systemic sclerosis. | Hudson, M., et al. 2012. J Rheumatol. 39: 787-94. PMID: 22467948
- Stable complex formation of CENP-B with the CENP-A nucleosome. | Fujita, R., et al. 2015. Nucleic Acids Res. 43: 4909-22. PMID: 25916850
- CENP-B protects centromere chromatin integrity by facilitating histone deposition via the H3.3-specific chaperone Daxx. | Morozov, VM., et al. 2017. Epigenetics Chromatin. 10: 63. PMID: 29273057
- CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly. | Otake, K., et al. 2020. J Cell Sci. 133: PMID: 32661090
- CENP-B promotes the centromeric localization of ZFAT to control transcription of noncoding RNA. | Ishikura, S., et al. 2021. J Biol Chem. 297: 101213. PMID: 34547289
- Increased Autoantibodies Against Ro/SS-A, CENP-B, and La/SS-B in Patients With Kidney Allograft Antibody-mediated Rejection. | Clotet-Freixas, S., et al. 2021. Transplant Direct. 7: e768. PMID: 34557585
- CENP-B-mediated DNA loops regulate activity and stability of human centromeres. | Chardon, F., et al. 2022. Mol Cell. 82: 1751-1767.e8. PMID: 35320753
- Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation. | Okazaki, K., et al. 2022. Cells. 11: PMID: 35563684
- CENP-A and CENP-B collaborate to create an open centromeric chromatin state. | Nagpal, H., et al. 2023. Nat Commun. 14: 8227. PMID: 38086807
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CENP-B Antibody (2D-7) | sc-32285 | 200 µg/ml | $322.00 | |||
CENP-B Antibody (2D-7): m-IgG Fc BP-HRP Bundle | sc-536731 | 200 µg Ab; 10 µg BP | $361.00 | |||
CENP-B Antibody (2D-7): m-IgGκ BP-HRP Bundle | sc-533892 | 200 µg Ab; 40 µg BP | $361.00 | |||
CENP-B Antibody (2D-7): m-IgG1 BP-HRP Bundle | sc-544787 | 200 µg Ab; 20 µg BP | $361.00 |