CENP-B Antibody (F-4): sc-376283

CENP-B Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CENP-B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CENP-B antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CENP-B protein plays a crucial role in the structure and function of centromeres, which are essential for accurate chromosome segregation during cell division. Specifically, CENP-B binds to centromeric alpha-satellite DNA, facilitating the formation of higher-order chromatin structures that are vital for maintaining centromere integrity. This binding not only protects DNA from nuclease digestion but also ensures proper assembly of the kinetochore, the protein complex that mediates spindle fiber attachment to chromosomes. CENP-B presence is critical for centromere stability, and dysfunction can lead to chromosomal instability, often associated with various cancers and genetic disorders. Using CENP-B monoclonal antibody (F-4) in research can provide valuable insights into chromosome dynamics and centromere protein roles in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CENP-B Antibody (F-4) References:

1. Characterization of a novel kinetochore protein, CENP-H.  |  Sugata, N., et al. 1999. J Biol Chem. 274: 27343-6. PMID: 10488063
2. Centromerization.  |  Choo, KH. 2000. Trends Cell Biol. 10: 182-8. PMID: 10754560
3. CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA.  |  Ohzeki, J., et al. 2002. J Cell Biol. 159: 765-75. PMID: 12460987
4. CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization.  |  Suzuki, N., et al. 2004. J Biol Chem. 279: 5934-46. PMID: 14612452
5. Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0.  |  Lomonte, P. and Morency, E. 2007. FEBS Lett. 581: 658-62. PMID: 17258208
6. Dynamics of satellite binding protein CENP-B and telomere binding protein TRF2/MTBP in the nuclei of mouse spermatogenic line.  |  Dolnik, AV., et al. 2007. Cell Biol Int. 31: 316-29. PMID: 17353134
7. CENP-B: a major human centromere protein located beneath the kinetochore.  |  Cooke, CA., et al. 1990. J Cell Biol. 110: 1475-88. PMID: 2335558
8. Identification of novel α-n-methylation of CENP-B that regulates its binding to the centromeric DNA.  |  Dai, X., et al. 2013. J Proteome Res. 12: 4167-75. PMID: 23978223
9. The fission yeast CENP-B protein Abp1 prevents pervasive transcription of repetitive DNA elements.  |  Daulny, A., et al. 2016. Biochim Biophys Acta. 1859: 1314-21. PMID: 27345571
10. Localization of the centromere protein CENP-B using scleroderma sera and evidence for a role in centromere survival.  |  Barbosa-Cisneros, O., et al. 1998. Rev Rhum Engl Ed. 65: 15-20. PMID: 9523381
11. The vertebrate cell kinetochore and its roles during mitosis.  |  Rieder, CL. and Salmon, ED. 1998. Trends Cell Biol. 8: 310-8. PMID: 9704407
12. In vitro assembly of the CENP-B/alpha-satellite DNA/core histone complex: CENP-B causes nucleosome positioning.  |  Yoda, K., et al. 1998. Genes Cells. 3: 533-48. PMID: 9797455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CENP-B Antibody (F-4) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CENP-B Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CENP-B Antibody (F-4) Specifications

Alternate Product Name: Centromere Protein B

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 535-599

Epitope Target: CENP-B

Epitope Species: human

Product Reactivity/Detection: CENP-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CENP-B Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin