Cdk5 Antibody (J-3): sc-6247

Cdk5 Antibody (J-3) is a mouse monoclonal IgG2b kappa light chain antibody that targets cyclin dependent kinase 5 (Cdk5), also known as PSSALRE, a critical protein involved in regulating the cell cycle and neuronal function. Cdk5 monoclonal antibody (J-3) is raised against amino acids 1-291 of the full-length human Cdk5 protein and demonstrates reactivity with Cdk5 from mouse, rat, and human species, making Cdk5 (J-3) antibody ideal for applications such as western blotting, immunoprecipitation, immunofluorescence, and ELISA. Cdk5 plays a pivotal role in the G1-S transition of the cell cycle and is predominantly expressed in mature neurons, where activity significantly increases during neuronal differentiation. Cdk5 is essential for phosphorylating key substrates like neurofilament and tau proteins, which are vital for maintaining neuronal structure and function. By modulating these processes, Cdk5 is integral to both normal cellular operations and the development of neurological disorders. Cdk5 monoclonal antibody (J-3) is available in various forms, including non-conjugated and multiple conjugated versions such as agarose, HRP, PE, FITC, and several Alexa Fluor® conjugates, providing researchers with flexible options for experimental needs. Cdk5 (J-3) antibody allows for precise detection and study of Cdk5, facilitating advancements in understanding its role in cell cycle regulation and neurobiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdk5 Antibody (J-3) References:

1. PCTAIRE-1 and PCTAIRE-3, two members of a novel cdc2/CDC28-related protein kinase gene family.  |  Okuda, T., et al. 1992. Oncogene. 7: 2249-58. PMID: 1437147
2. The in vivo phosphorylation sites of rat brain dynamin I.  |  Graham, ME., et al. 2007. J Biol Chem. 282: 14695-707. PMID: 17376771
3. CDK5: A Unique CDK and Its Multiple Roles in the Nervous System.  |  Cortés, N., et al. 2019. J Alzheimers Dis. 68: 843-855. PMID: 30856110
4. CDK5: Key Regulator of Apoptosis and Cell Survival.  |  Roufayel, R. and Murshid, N. 2019. Biomedicines. 7: PMID: 31698798
5. CDK5: an oncogene or an anti-oncogene: location location location.  |  Nikhil, K. and Shah, K. 2023. Mol Cancer. 22: 186. PMID: 37993880
6. The cell cycle kinases.  |  Pines, J. 1994. Semin Cancer Biol. 5: 305-13. PMID: 7803767
7. Synergistic gene expressions of cyclin E, cdk2, cdk5 and E2F-1 during the prolactin-induced G1/S transition in rat Nb2 pre-T lymphoma cells.  |  Hosokawa, Y., et al. 1995. Biochem Mol Biol Int. 37: 393-9. PMID: 8673024
8. Cyclins, cyclin-dependent kinases and cdk inhibitors: implications in cell cycle control and cancer.  |  MacLachlan, TK., et al. 1995. Crit Rev Eukaryot Gene Expr. 5: 127-56. PMID: 8845581
9. Intracellular localization of cyclin-dependent kinase 5 (CDK5) in mouse neuron: CDK5 is located in both nucleus and cytoplasm.  |  Ino, H. and Chiba, T. 1996. Brain Res. 732: 179-85. PMID: 8891282
10. cdk5 expression and association with p35nck5a in early stages of rat cerebellum neurogenesis; tyrosine dephosphorylation and activation in post-mitotic neurons.  |  Lazaro, JB., et al. 1996. Neurosci Lett. 218: 21-4. PMID: 8939471
11. Role of the cyclin-dependent kinase 4 and 6 inhibitor gene family p15, p16, p18 and p19 in leukemia and lymphoma.  |  Siebert, R., et al. 1996. Leuk Lymphoma. 23: 505-20. PMID: 9031081
12. Current concepts in neuro-oncology: the cell cycle--a review.  |  Dirks, PB. and Rutka, JT. 1997. Neurosurgery. 40: 1000-13; discussion 1013-5. PMID: 9149259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdk5 Antibody (J-3) has 130 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdk5 Antibody (J-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdk5 Antibody (J-3) Specifications

Alternate Product Name: Cdk5

Product Clone Name: J-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-291

Epitope Target: Cdk5

Epitope Species: human

Product Reactivity/Detection: Cdk5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 130 citations in various scientific publications

Available Conjugates/Forms for Cdk5 Antibody (J-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine

The preferred Cdk5 antibody is Cdk5 Antibody (DC 17): sc-249.