CDK2AP1 Antibody (H-4): sc-390283
- CDK2AP1 Antibody (H-4) is a mouse monoclonal IgG1 κ CDK2AP1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 20-76 mapping within an internal region of CDK2AP1 of human origin
- CDK2AP1 Antibody (H-4) is recommended for detection of CDK2AP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CDK2AP1 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CDK2AP1 Antibody (H-4) for WB applications. These reagents are now offered in bundles with CDK2AP1 Antibody (H-4) (see ordering information below).
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CDK2AP1 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CDK2AP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CDK2AP1 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CDK2AP1, also known as p12 or DOC-1, plays a crucial role as a growth suppressor by binding to and inhibiting DNA polymerase α/primase, which is essential for DNA replication initiation. Such inhibition prevents unwarranted cell cycle progression, maintaining genomic stability. CDK2AP1 also interacts with cyclin-dependent kinase 2 (Cdk2) and targets Cdk2 for proteolysis, promoting cell cycle arrest during S phase and potentially triggering apoptosis. TGFβ1 transcriptionally induces CDK2AP1 expression, mediating growth inhibitory effects by modulating Cdk2 activities and retinoblastoma protein (pRB) phosphorylation. Due to CDK2AP1′s significant role in regulating cell proliferation and apoptosis, researchers consider CDK2AP1 a promising candidate for tumor suppression, particularly in oral cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CDK2AP1 Antibody (H-4) References:
- p12(DOC-1), a growth suppressor, associates with DNA polymerase alpha/primase. | Matsuo, K., et al. 2000. FASEB J. 14: 1318-24. PMID: 10877824
- p12(DOC-1) is a novel cyclin-dependent kinase 2-associated protein. | Shintani, S., et al. 2000. Mol Cell Biol. 20: 6300-7. PMID: 10938106
- Role of p12(CDK2-AP1) in transforming growth factor-beta1-mediated growth suppression. | Hu, MG., et al. 2004. Cancer Res. 64: 490-9. PMID: 14744761
- Deleted in oral cancer-1 expression upregulates proapoptosis elements in microsatellite-unstable human colorectal cancer. | Sotsky Kent, T., et al. 2004. Ann Surg Oncol. 11: 192-6. PMID: 14761923
- Interaction of the CDK2-associated protein-1, p12(DOC-1/CDK2AP1), with its homolog, p14(DOC-1R). | Buajeeb, W., et al. 2004. Biochem Biophys Res Commun. 315: 998-1003. PMID: 14985111
- p12(CDK2-AP1) mediates DNA damage responses induced by cisplatin. | Kim, Y., et al. 2005. Oncogene. 24: 407-18. PMID: 15543230
- Expression patterns of mitotic and meiotic cell cycle regulators in testicular cancer and development. | Diederichs, S., et al. 2005. Int J Cancer. 116: 207-17. PMID: 15800920
- Modulation of CDK2-AP1 (p12(DOC-1)) expression in human colorectal cancer. | Yuan, Z., et al. 2005. Oncogene. 24: 3657-68. PMID: 15806176
- Cloning, mapping, expression, function, and mutation analyses of the human ortholog of the hamster putative tumor suppressor gene Doc-1. | Tsuji, T., et al. 1998. J Biol Chem. 273: 6704-9. PMID: 9506968
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDK2AP1 Antibody (H-4) | sc-390283 | 200 µg/ml | $322.00 | |||
CDK2AP1 Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-527621 | 200 µg Ab; 10 µg BP | $361.00 | |||
CDK2AP1 Antibody (H-4): m-IgG1 BP-HRP Bundle | sc-532994 | 200 µg Ab; 20 µg BP | $361.00 | |||
CDK2AP1 Antibody (H-4) AC | sc-390283 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CDK2AP1 Antibody (H-4) HRP | sc-390283 HRP | 200 µg/ml | $322.00 | |||
CDK2AP1 Antibody (H-4) FITC | sc-390283 FITC | 200 µg/ml | $336.00 | |||
CDK2AP1 Antibody (H-4) PE | sc-390283 PE | 200 µg/ml | $349.00 | |||
CDK2AP1 Antibody (H-4) Alexa Fluor® 488 | sc-390283 AF488 | 200 µg/ml | $364.00 | |||
CDK2AP1 Antibody (H-4) Alexa Fluor® 546 | sc-390283 AF546 | 200 µg/ml | $364.00 | |||
CDK2AP1 Antibody (H-4) Alexa Fluor® 594 | sc-390283 AF594 | 200 µg/ml | $364.00 | |||
CDK2AP1 Antibody (H-4) Alexa Fluor® 647 | sc-390283 AF647 | 200 µg/ml | $364.00 | |||
CDK2AP1 Antibody (H-4) Alexa Fluor® 680 | sc-390283 AF680 | 200 µg/ml | $364.00 | |||
CDK2AP1 Antibody (H-4) Alexa Fluor® 790 | sc-390283 AF790 | 200 µg/ml | $364.00 |