Date published: 2026-5-21

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Cdc6 Antibody (D-1): sc-13136

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Datasheets
  • Cdc6 Antibody (D-1) is a mouse monoclonal IgG1 κ, cited in 12 publications, provided at 200 µg/ml
  • raised against amino acids 257-560 of Cdc6 of human origin
  • Cdc6 Antibody (D-1) is recommended for detection of Cdc6 of human origin by WB, IP, IF and ELISA
  • Anti-Cdc6 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdc6 Antibody (D-1) for WB applications. These reagents are now offered in bundles with Cdc6 Antibody (D-1) (see ordering information below).
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Cdc6 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc6 of human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdc6 monoclonal antibody (D-1) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc6 plays a crucial role in the initiation of DNA replication, which is essential for cell division and proper progression of the cell cycle. Cdc6 helps maintain genomic stability and prevents errors that could lead to cell malfunction or disease by ensuring DNA replication occurs at the right time. Cdc6 interacts with several key proteins involved in cell cycle regulation, including cyclin-dependent kinases and various ubiquitin-conjugating enzymes, which are vital for transition between different phases of the cell cycle. Regulation of these interactions ensures cells replicate DNA accurately and divide correctly, thereby supporting overall cellular health and function.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cdc6 Antibody (D-1)

sc-13136
200 µg/ml
$322.00

Cdc6 Antibody (D-1): m-IgG Fc BP-HRP Bundle

sc-526539
200 µg Ab; 10 µg BP
$361.00

Cdc6 Antibody (D-1): m-IgG1 BP-HRP Bundle

sc-531912
200 µg Ab; 20 µg BP
$361.00

Cdc6 Antibody (D-1) AC

sc-13136 AC
500 µg/ml, 25% agarose
$424.00

Cdc6 Antibody (D-1) HRP

sc-13136 HRP
200 µg/ml
$322.00

Cdc6 Antibody (D-1) FITC

sc-13136 FITC
200 µg/ml
$336.00

Cdc6 Antibody (D-1) PE

sc-13136 PE
200 µg/ml
$349.00

Cdc6 Antibody (D-1) Alexa Fluor® 488

sc-13136 AF488
200 µg/ml
$364.00

Cdc6 Antibody (D-1) Alexa Fluor® 546

sc-13136 AF546
200 µg/ml
$364.00

Cdc6 Antibody (D-1) Alexa Fluor® 594

sc-13136 AF594
200 µg/ml
$364.00

Cdc6 Antibody (D-1) Alexa Fluor® 647

sc-13136 AF647
200 µg/ml
$364.00

Cdc6 Antibody (D-1) Alexa Fluor® 680

sc-13136 AF680
200 µg/ml
$364.00

Cdc6 Antibody (D-1) Alexa Fluor® 790

sc-13136 AF790
200 µg/ml
$364.00

What is the recommended fixation method for immunofluorescence staining with Cdc6 (D-1): sc-13136 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody for Western blotThis antibody performs very well for Western blot for multiple human cell lines.
Date published: 2020-01-05
Rated 1 out of 5 by from CDC6 AbThe antibody didn´t give me any signal using HCT116 nuclear extracts performed by standard WB procedure that worked for another Santa Cruz antibodies (for example sc-9964 – also against CDC6).
Date published: 2018-07-23
Rated 5 out of 5 by from Excellent antibody! Published for ChIP.This monoclonal Cdc6 Antibody (D-1) has excellent in-house data for Western blot and IF, and it has been published for ChIP (see PMID: 26872786).
Date published: 2017-02-07
Rated 5 out of 5 by from good antibodygood antibody, used in WB on human samples
Date published: 2016-07-25
Rated 4 out of 5 by from This antibody works well and can be usedThis antibody works well and can be used for IP and Western Blot with human HeLa, T98G, U2-OS, HEK 293T, H1299 cell lines. -SCBT Publication Review
Date published: 2015-04-29
Rated 5 out of 5 by from Works well in Western Blot application withWorks well in Western Blot application with the human HEF cell line. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from Strong band at correct molecular weight observedStrong band at correct molecular weight observed by Western Blot with HeLa nuclear extract. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Produced good quality flow cytometry dataProduced good quality flow cytometry data in HeLa and S3 cells. -SCBT Publication Review
Date published: 2015-01-09
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Cdc6 Antibody (D-1) is rated 4.3 out of 5 by 9.
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