Cdc6 Antibody (180.2) recommended for detection of Cdc6 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Cdc6 Antibody (180.2): sc-9964

Cdc6 Antibody (180.2) is rated 4.9 out of 5 by 14.
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Datasheets

    • Anti-Cdc6 Antibody (180.2) is a mouse monoclonal IgG1 (kappa light chain) Cdc6 antibody provided at 200 µg/ml
    • raised against full length Cdc6 of human origin
    • Anti-Cdc6 Antibody (180.2) is recommended for detection of Cdc6 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Cdc6 Antibody (180.2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc6 (180.2): sc-9964.
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Thanks. I'm working on paraffin-embedded mouse livers. The tissue has proven troublesome in the past (i.e. antibodies working just fine on other paraffin tissues not working at all). Any particular suggestion (i.e. harsher retrieval conditions)? 

Asked by: alberto grn
Thank you for your question. We do not have a specific protocol for mouse liver, but here is a link to our suggested protocol: https://www.scbt.com/resources/protocols/immunoperoxidase-staining We recommend sodium citrate with heat as the primary method, but you can also try any of the other methods in the protocol.
Answered by: Tech Service
Date published: 2019-10-23

Is the antibody suitable for immunofluorescence from paraffin-embedded tissues? If so, what antigen retrieval protocol do you recommend?  Thanks in advance.

Asked by: alberto grn
Thank you for your question. Yes, this antibody, sc-9964, Cdc6 (180.2) is recommended for IF/IHC on paraffin embedded tissue sections. We recommend a starting dilution of 1:50, and suggest antigen retrieval using sodium citrate with heat. You can find the recommended protocol here: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-10-22

The antibody sc9964 recognize which part of Cdc6, what is the meaning of 180.2, thank you !

Asked by: LiuLiuM
Thank you for your question. The exact epitope recognized by this monoclonal antibody has not been determined. And 180.2 is the clone designation for this antibody.
Answered by: Technical Support
Date published: 2019-01-26

How can Cdc6 Antibody (180.2): sc-9964 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available phycoerythrin (sc-9964 PE), fluorescein (sc-9964 FITC), Alexa Fluor® 488 (sc-9964 AF488) or Alexa Fluor® 647 (sc-9964 AF647).
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 5 out of 5 by from Good antibody We used this antibody in WB and got good signal, no complaints.
Date published: 2018-02-19
Rated 5 out of 5 by from Very Specific Antibody! Worked great on cell lysates! Detected signal in untreated and was upregulated in treated cells.
Date published: 2018-01-31
Rated 4 out of 5 by from Good for western Detected a predicted size band in mouse tissue samples
Date published: 2017-09-29
Rated 5 out of 5 by from good for WB i recieved this antibody 2 momths ago for free trial, and i'm happy to got a band in the right place (1:1000 dilution),the band is strong
Date published: 2017-08-15
Rated 4 out of 5 by from Faint but distinct bands on WB Produced faint but distinct bands on WB. used the ab at 1:1000 dilution
Date published: 2017-07-03
Rated 5 out of 5 by from Good results We use this product the WB dilution of 1:500 had a strong signal.
Date published: 2017-04-10
Rated 5 out of 5 by from Wow !! Outstanding For all applications including western, IHC and IF best cdc6 antibody
Date published: 2017-01-07
Rated 5 out of 5 by from Great for staining Equally specific results in IHC(P) and in immunofluorescenc.
Date published: 2016-12-23
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