Cdc6 Antibody (0.N.189) recommended for detection of Cdc6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cdc6 Antibody (0.N.189): sc-70827

Cdc6 Antibody (0.N.189) is rated 3.0 out of 5 by 1.
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Datasheets

    • Cdc6 Antibody (0.N.189) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length Cdc6 of human origin
    • recommended for detection of Cdc6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Cdc6 (180.2): sc-9964 for Cdc6 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

How much sample do you recommend loading on the gel for Western blot detection using Cdc6 (0.N.189): sc-70827?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Strong signal with some background for western Strong signal with some background for western blot analysis of Cdc6 expression in non-transfected and human Cdc6 transfected 293T whole cell lysates. - SCBT QC
Date published: 2014-08-21
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