Cdc37 Antibody (C-11) recommended for detection of Cdc37 of human origin by WB, IP, IF, IHC(P) and ELISA

Cdc37 Antibody (C-11): sc-17758

Cdc37 Antibody (C-11) is rated 5.0 out of 5 by 5.
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Datasheets

    • Cdc37 Antibody (C-11) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 108-378 of Cdc37 of human origin
    • recommended for detection of Cdc37 of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using Cdc37 (C-11): sc-17758 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong cytoplasmic localization in IF in methanol Strong cytoplasmic localization in IF in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from Worked well in IP and WB in HEK293 and RT112 Worked well in IP and WB in HEK293 and RT112 cells. -SCBT Publication Review
Date published: 2015-03-01
Rated 5 out of 5 by from Good specificity in WB in Jurkat and K Good specificity in WB in Jurkat and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-13
Rated 5 out of 5 by from Good antibody for formalin fixed human duodenum Good antibody for formalin fixed human duodenum tissue showing cytoplasmic and membrane staining in IHC. -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Good WB antibdy in COS7 transfected with wild Good WB antibdy in COS7 transfected with wild-type PINK1-FLAG or L347P mutant PINK1-FLAG. -SCBT Publication Review
Date published: 2013-02-28
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