Cdc25C Antibody (F-5) recommended for detection of Cdc25C of human origin by WB, IP, IF and ELISA

Cdc25C Antibody (F-5): sc-55513

Cdc25C Antibody (F-5) is rated 5.0 out of 5 by 4.
  • y_2020, m_4, d_7, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_55513, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 158ms
  • REVIEWS, PRODUCT
5
4
4
0
3
0
2
0
1
0

Datasheets

    • Cdc25C Antibody (F-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-150 mapping at the N-terminus of Cdc25C of human origin
    • recommended for detection of Cdc25C of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Cdc25C (H-6): sc-13138 for Cdc25C antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
Submit a review for this product and receive 15 CruzCredits

What is the recommended starting dilution for Western blot for sc-55513: Cdc25C (F-5) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
  • y_2020, m_4, d_7, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_55513, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 131ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good publishable results by Western Blot Good publishable results by Western Blot obtained using mouse embryo cortex and A7 melanoma samples. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by from Using immunofluorescence Using immunofluorescence, good staining observed for mouse embryo cortex and A7 melanoma samples. -SCBT Publication Review
Date published: 2015-03-27
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the Cdc25C protein via Western Blot analysis. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Robust signal obtained at appropriate molecular Robust signal obtained at appropriate molecular weight via Western Blot using K-562 and Raji whole cell lysates. -SCBT QC
Date published: 2014-02-05
  • y_2020, m_4, d_7, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_55513, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 22ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.