Cdc123 Antibody (A-2): sc-390989
- Cdc123 Antibody (A-2) is a mouse monoclonal IgG2b κ Cdc123 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 289-318 near the C-terminus of Cdc123 of human origin
- Cdc123 Antibody (A-2) is recommended for detection of Cdc123 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Cdc123 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc123 Antibody (A-2) for WB applications. These reagents are now offered in bundles with Cdc123 Antibody (A-2) (see ordering information below).
Cdc123 Antibody (A-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Cdc123 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Cdc123 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdc123, also known as cell division cycle protein 123 or D123, is a crucial 336 amino acid cytoplasmic protein that plays a significant role in regulating the cell cycle. Cdc123 is widely expressed in tissues such as thymus, spleen, ovary, testis, small intestine, and leukocytes, highlighting its importance in cellular functions. Cdc123 is essential for preventing accumulation of checkpoint with forkhead and ring finger domain (Chfr) proteins, which can inhibit transition from G1 to S phase of the cell cycle. By destabilizing Chfr proteins, Cdc123 facilitates progression of cells into S phase, thereby promoting proper cell division and growth. Given its pivotal role in cell cycle regulation, Cdc123 is considered a potential basal marker for luminal breast cancers, making Cdc123 (A-2) monoclonal antibody a valuable tool for researchers studying cancer biology and cell cycle dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc123 Antibody (A-2) References:
- Extensive degradation of mutant-type D123 protein is responsible for temperature-sensitive proliferation inhibition in 3Y1tsD123 cells. | Okuda, A., et al. 1999. Cell Struct Funct. 24: 443-9. PMID: 10698258
- Mutation of a conserved residue (D123) required for oligomerization of human immunodeficiency virus type 1 Nef protein abolishes interaction with human thioesterase and results in impairment of Nef biological functions. | Liu, LX., et al. 2000. J Virol. 74: 5310-9. PMID: 10799608
- Reversion of temperature-sensitive mutation by inhibition of proteasome-mediated degradation of mutated D123 protein. | Okuda, A., et al. 2001. Cell Struct Funct. 26: 205-14. PMID: 11699637
- Cdc123 and checkpoint forkhead associated with RING proteins control the cell cycle by controlling eIF2gamma abundance. | Bieganowski, P., et al. 2004. J Biol Chem. 279: 44656-66. PMID: 15319434
- Integrated profiling of basal and luminal breast cancers. | Adélaïde, J., et al. 2007. Cancer Res. 67: 11565-75. PMID: 18089785
- Meta-analysis of genome-wide association data and large-scale replication identifies additional susceptibility loci for type 2 diabetes. | Zeggini, E., et al. 2008. Nat Genet. 40: 638-45. PMID: 18372903
- Association testing of novel type 2 diabetes risk alleles in the JAZF1, CDC123/CAMK1D, TSPAN8, THADA, ADAMTS9, and NOTCH2 loci with insulin release, insulin sensitivity, and obesity in a population-based sample of 4,516 glucose-tolerant middle-aged Danes. | Grarup, N., et al. 2008. Diabetes. 57: 2534-40. PMID: 18567820
- An amino acid change in novel protein D123 is responsible for temperature-sensitive G1-phase arrest in a mutant of rat fibroblast line 3Y1. | Okuda, A. and Kimura, G. 1996. Exp Cell Res. 223: 242-9. PMID: 8601400
- Expression study on D123 gene product: evidence for high positivity in testis. | Onisto, M., et al. 1998. Exp Cell Res. 242: 451-9. PMID: 9683532
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc123 Antibody (A-2) | sc-390989 | 200 µg/ml | $316.00 | |||
Cdc123 Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-523765 | 200 µg Ab, 40 µg BP | $354.00 | |||
Cdc123 Antibody (A-2): m-IgG2b BP-HRP Bundle | sc-549137 | 200 µg Ab; 10 µg BP | $354.00 | |||
Cdc123 Antibody (A-2) AC | sc-390989 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Cdc123 Antibody (A-2) HRP | sc-390989 HRP | 200 µg/ml | $316.00 | |||
Cdc123 Antibody (A-2) FITC | sc-390989 FITC | 200 µg/ml | $330.00 | |||
Cdc123 Antibody (A-2) PE | sc-390989 PE | 200 µg/ml | $343.00 | |||
Cdc123 Antibody (A-2) Alexa Fluor® 488 | sc-390989 AF488 | 200 µg/ml | $357.00 | |||
Cdc123 Antibody (A-2) Alexa Fluor® 546 | sc-390989 AF546 | 200 µg/ml | $357.00 | |||
Cdc123 Antibody (A-2) Alexa Fluor® 594 | sc-390989 AF594 | 200 µg/ml | $357.00 | |||
Cdc123 Antibody (A-2) Alexa Fluor® 647 | sc-390989 AF647 | 200 µg/ml | $357.00 | |||
Cdc123 Antibody (A-2) Alexa Fluor® 680 | sc-390989 AF680 | 200 µg/ml | $357.00 | |||
Cdc123 Antibody (A-2) Alexa Fluor® 790 | sc-390989 AF790 | 200 µg/ml | $357.00 | |||
Cdc123 (A-2) Neutralizing Peptide | sc-390989 P | 100 µg/0.5 ml | $68.00 |