CD8-β Antibody (H35-17.2): sc-20041

CD8-β Antibody (H35-17.2) is a rat monoclonal IgG2b antibody that detects CD8 in mouse samples through applications such as immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD8 is a crucial cell surface glycoprotein that forms a heterodimeric complex, typically composed of either αα or αβ chains, and plays a vital role in the immune response by binding to class I major histocompatibility complex (MHC) molecules presented by antigen-presenting cells. This interaction is essential for T cytotoxic cells, which express CD8, as CD8 enhances the recognition of antigens by the T cell receptor (TCR) and strengthens the avidity of TCR-antigen interactions, thereby facilitating effective immune responses. Additionally, CD8-expressing T cells are implicated in maintaining low levels of HIV expression, highlighting CD8′s importance not only in adaptive immunity but also in viral control. Anti-CD8-β antibody (H35-17.2) is an invaluable tool for researchers studying T cell biology, immune responses, and the mechanisms of viral infections, providing insights into the complex interactions that govern immune system function.

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CD8-β Antibody (H35-17.2) References:

1. Human CD8 beta, but not mouse CD8 beta, can be expressed in the absence of CD8 alpha as a beta beta homodimer.  |  Devine, L., et al. 2000. J Immunol. 164: 833-8. PMID: 10623829
2. Generation of anti-human CD8 beta-specific antibodies using transfectants expressing mixed-species CD8 heterodimers.  |  DiSanto, JP., et al. 1991. J Immunol Methods. 141: 123-31. PMID: 1830894
3. Comparative analysis of CD8 expressed on mature CD4+ CD8+ T cell clones cultured with IL-4 and that on CD8+ T cell clones: implication for functional significance of CD8 beta.  |  Hori, T., et al. 1991. Int Immunol. 3: 737-41. PMID: 1911543
4. Differential expression and regulation of the human CD8 alpha and CD8 beta chains.  |  Terry, LA., et al. 1990. Tissue Antigens. 35: 82-91. PMID: 2111591
5. CD8 beta chain influences CD8 alpha chain-associated Lck kinase activity.  |  Irie, HY., et al. 1995. J Exp Med. 181: 1267-73. PMID: 7699318
6. Transcriptional diversity at the duplicated human CD8 beta loci.  |  DiSanto, JP., et al. 1993. Eur J Immunol. 23: 320-6. PMID: 8436166
7. CD8 beta increases CD8 coreceptor function and participation in TCR-ligand binding.  |  Renard, V., et al. 1996. J Exp Med. 184: 2439-44. PMID: 8976201
8. Expression of CD8beta and alteration of cell surface phenotype in adult T-cell leukaemia cells.  |  Joh, T., et al. 1997. Br J Haematol. 98: 151-6. PMID: 9233579
9. Co-capping studies reveal CD8/TCR interactions after capping CD8 beta polypeptides and intracellular associations of CD8 with p56(lck).  |  Kwan Lim, GE., et al. 1998. Eur J Immunol. 28: 745-54. PMID: 9521084
10. Mechanisms of CD8beta-mediated T cell response enhancement: interaction with MHC class I/beta2-microglobulin and functional coupling to TCR/CD3.  |  Wheeler, CJ., et al. 1998. J Immunol. 160: 4199-207. PMID: 9574520

The preferred CD8-β antibody is CD8-β Antibody (F-5): sc-25277.