CD8 Antibody (32-M4): sc-1177
- CD8 Antibody (32-M4) is a mouse monoclonal IgG2a κ CD8 antibody, cited in 31 publications, provided at 200 µg/ml
- raised against CD8 of human origin
- CD8 Antibody (32-M4) is recommended for detection of CD8 of mouse, rat and human origin by WB, IP, IF and FCM
- Anti-CD8 Antibody (32-M4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to APC for IF, IHC(P) and FCM
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD8 Antibody (32-M4) for WB applications. These reagents are now offered in bundles with CD8 Antibody (32-M4) (see ordering information below).
CD8 Antibody (32-M4) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-CD8 antibody (32-M4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD8 protein, also known as Leu 2 or T8, is a crucial cell surface glycoprotein that forms a two-chain complex (either αα or αβ) and plays a vital role in the immune response by binding to class I major histocompatibility complex (MHC) molecules presented by antigen-presenting cells (APCs). This interaction is essential for the activation of T cytotoxic cells, which are responsible for directly killing infected or cancerous cells. CD8 enhances the avidity of T cell receptor (TCR) interactions with antigens, thereby facilitating effective immune responses. CD8-expressing T cells may contribute to the maintenance of low levels of HIV expression, highlighting CD8′s importance in both adaptive immunity and viral control.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD8 Antibody (32-M4) References:
- Potentiating the antitumour response of CD8(+) T cells by modulating cholesterol metabolism. | Yang, W., et al. 2016. Nature. 531: 651-5. PMID: 26982734
- Epigenetic control of CD8+ T cell differentiation. | Henning, AN., et al. 2018. Nat Rev Immunol. 18: 340-356. PMID: 29379213
- Cholesterol Induces CD8+ T Cell Exhaustion in the Tumor Microenvironment. | Ma, X., et al. 2019. Cell Metab. 30: 143-156.e5. PMID: 31031094
- TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. | Khan, O., et al. 2019. Nature. 571: 211-218. PMID: 31207603
- The Roles of CD8+ T Cell Subsets in Antitumor Immunity. | St Paul, M. and Ohashi, PS. 2020. Trends Cell Biol. 30: 695-704. PMID: 32624246
- Memory CD8+ T cell responses to cancer. | Han, J., et al. 2020. Semin Immunol. 49: 101435. PMID: 33272898
- Systemic vaccination induces CD8+ T cells and remodels the tumor microenvironment. | Baharom, F., et al. 2022. Cell. 185: 4317-4332.e15. PMID: 36302380
- CD8+ T cell exhaustion and cancer immunotherapy. | Wang, Q., et al. 2023. Cancer Lett. 559: 216043. PMID: 36584935
- Regulation of CD8+ T memory and exhaustion by the mTOR signals. | Chen, Y., et al. 2023. Cell Mol Immunol. 20: 1023-1039. PMID: 37582972
- Circadian tumor infiltration and function of CD8+ T cells dictate immunotherapy efficacy. | Wang, C., et al. 2024. Cell. 187: 2690-2702.e17. PMID: 38723627
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD8 Antibody (32-M4) | sc-1177 | 200 µg/ml | $322.00 | |||
CD8 Antibody (32-M4): m-IgG Fc BP-HRP Bundle | sc-553326 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD8 Antibody (32-M4): m-IgGκ BP-HRP Bundle | sc-553325 | 200 µg Ab; 40 µg BP | $361.00 | |||
CD8 Antibody (32-M4): m-IgG2a BP-HRP Bundle | sc-553327 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD8 Antibody (32-M4) AC | sc-1177 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD8 Antibody (32-M4) HRP | sc-1177 HRP | 200 µg/ml | $322.00 | |||
CD8 Antibody (32-M4) FITC | sc-1177 FITC | 200 µg/ml | $336.00 | |||
CD8 Antibody (32-M4) PE | sc-1177 PE | 200 µg/ml | $349.00 | |||
CD8 Antibody (32-M4) Alexa Fluor® 488 | sc-1177 AF488 | 200 µg/ml | $364.00 | |||
CD8 Antibody (32-M4) Alexa Fluor® 546 | sc-1177 AF546 | 200 µg/ml | $364.00 | |||
CD8 Antibody (32-M4) Alexa Fluor® 594 | sc-1177 AF594 | 200 µg/ml | $364.00 | |||
CD8 Antibody (32-M4) Alexa Fluor® 647 | sc-1177 AF647 | 200 µg/ml | $364.00 | |||
CD8 Antibody (32-M4) Alexa Fluor® 680 | sc-1177 AF680 | 200 µg/ml | $364.00 | |||
CD8 Antibody (32-M4) Alexa Fluor® 790 | sc-1177 AF790 | 200 µg/ml | $364.00 | |||
CD8 Antibody (32-M4) APC | sc-1177 APC | 100 tests in 2 ml | $358.00 |