CD73 Antibody (D-12): sc-398260

CD73 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD73 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CD73 antibody (D-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD73, also known as ecto-5′-nucleotidase, plays a crucial role in purine metabolism by catalyzing the dephosphorylation of extracellular nucleotides into bioactive nucleosides, which are essential for various physiological processes, including immune response and tissue repair. Located on the external face of the plasma membrane, CD73 is a glycosyl-phosphatidylinositol (GPI)-anchored protein that forms a dimer of two identical subunits, allowing CD73 to mediate co-stimulatory signals during T cell activation. This unique structure and anchoring mechanism enable CD73 to interact with a variety of cell types, including endothelial cells and lymphocytes, thereby influencing immune cell adhesion and activation. Notably, modulation of CD73 activity can significantly impact lymphocyte behavior, as CD73 neutralization can disrupt lymphocyte adhesion to endothelial cells and affect the aggregation of germinal center B cells and follicular dendritic cells. This highlights CD73′s importance in maintaining immune homeostasis and potential as a therapeutic target in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD73 Antibody (D-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-CD73 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD73 Antibody (D-12) Specifications

Alternate Product Name: 5'-nucleotidase

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 275-574

Epitope Target: CD73

Epitope Species: human

Product Reactivity/Detection: CD73

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for CD73 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD73 antibody is CD73 Antibody (IE9): sc-32299.