CD73 Antibody (IE9) recommended for detection of CD73 of human origin by WB, IP, IF and IHC(P)

CD73 Antibody (IE9): sc-32299

CD73 Antibody (IE9) is rated 4.9 out of 5 by 8.
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Datasheets

    • CD73 Antibody (IE9) is a mouse monoclonal IgG3 κ, cited in 29 publications, provided at 200 µg/ml
    • raised against human CD73 purified from placenta
    • recommended for detection of CD73 of human origin by WB, IP, IF and IHC(P); non cross-reactive with mouse CD73
    • See CD73 (D-12): sc-398260 for CD73 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD73 (IE9): sc-32299.

Hi, I would like to know if it is possible to have the product sc-32299 in a carrier-free version (without gelatin)? Thank you

Asked by: Anonymous
Thank you for your question. Yes, this can be provided carrier-free (without gelatin) as a special order. Please contact our Special Orders Department to inquire about availability and to receive a quote. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2019-12-07

Hello: Was CD73 Antibody (IE9): sc-32299 tested on rat tissues? It was found to inhibit the catalytic activity of CD73 (in http://www.jimmunol.org/content/191/8/4165.long). This would be great if I could use it in rat tissue homogenates? thanks, Fran.

Asked by: FranVdB
Thank you for your question. We do not have any data indicating that this mouse monoclonal antibody, CD73 (IE9): sc-32299, is reactive with rat CD73. We recommend the antibody for detection of CD73 of human origin only. In the publication you have cited, the authors specifically mention that IE9 clone "failed to detect the murine CD73 variant ".
Answered by: Technical Support
Date published: 2017-06-28

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-23
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Rated 5 out of 5 by from Works well for human mesenchymal Stem cells Received sample for this 2 months ago. Worked pretty well with human BMMSCs at 1:50. Definitely going to purchase
Date published: 2020-03-10
Rated 4 out of 5 by from good used dilution 1: 150 overnight for immunofluorescence
Date published: 2018-04-10
Rated 5 out of 5 by from Works well in Western This is an excellent antibody for use in Western Blot - good band strength and clarity with little to no background.
Date published: 2016-12-27
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the CD73 protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Tested for western blotting with MB Tested for western blotting with MB-MDA-231, MB-MDA-453, MB-MDA-468, T47D, and MCF-7 cells, and breast cancer tissue extracts. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from IHC: acetone fixed ovarian cancer cryosections IHC: acetone fixed ovarian cancer cryosections. -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of 5 by from Clean band in human recombinant CD73 via Clean band in human recombinant CD73 via western blotting. -SCBT QC
Date published: 2015-01-23
Rated 5 out of 5 by from Published IHC data with human trabecular Published IHC data with human trabecular bone sections. -SCBT Publication Review
Date published: 2014-09-25
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