CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1): sc-32272

CD71 Antibody (3B8 2A1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD71 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD71 (3B8 2A1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD71 protein, also known as transferrin receptor (TFR), is a type II membrane glycoprotein that plays a crucial role in iron homeostasis by mediating cellular uptake of transferrin-bound iron through receptor-mediated endocytosis. This process is vital for maintaining adequate iron levels, which are essential for various cellular functions, including oxygen transport and DNA synthesis. CD71 is typically expressed at high levels on proliferating cells, reticulocytes, and erythroid precursors, while CD71 expression is upregulated in activated lymphocytes, monocytes, and macrophages. CD71 is also present on most dividing cells and on brain endothelium, highlighting CD71′s importance in both hematopoiesis and neurobiology. Additionally, a second transferrin receptor, TFR2, also facilitates uptake of transferrin-bound iron and is predominantly expressed in liver, where mutations in TFR2 gene can lead to hereditary hemochromatosis type III, an iron overload disorder.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1) References:

1. Recruitment of transferrin receptor to immunological synapse in response to TCR engagement.  |  Batista, A., et al. 2004. J Immunol. 172: 6709-14. PMID: 15153487
2. Differential expression and function of IgA receptors (CD89 and CD71) during maturation of dendritic cells.  |  Pasquier, B., et al. 2004. J Leukoc Biol. 76: 1134-41. PMID: 15371488
3. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action.  |  Kemp, JD., et al. 1989. Cell Immunol. 122: 218-30. PMID: 2526689
4. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols.  |  Kemp, JD., et al. 1987. J Immunol. 138: 2422-6. PMID: 2951439
5. Inhibition of cell growth by monoclonal anti-transferrin receptor antibodies.  |  Lesley, JF. and Schulte, RJ. 1985. Mol Cell Biol. 5: 1814-21. PMID: 3018527
6. ESR1 inhibits ionizing radiation-induced ferroptosis in breast cancer cells via the NEDD4L/CD71 pathway.  |  Liu, L., et al. 2022. Arch Biochem Biophys. 725: 109299. PMID: 35613689
7. Effects of monoclonal antibodies that block transferrin receptor function on the in vivo growth of a syngeneic murine leukemia.  |  Sauvage, CA., et al. 1987. Cancer Res. 47: 747-53. PMID: 3802079
8. CD71 expressing circulating neutrophils serve as a novel prognostic biomarker for metastatic spread and reduced outcome in pancreatic ductal adenocarcinoma patients.  |  Hansen, FJ., et al. 2024. Sci Rep. 14: 21164. PMID: 39256468
9. The human transferrin receptor gene: genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence.  |  McClelland, A., et al. 1984. Cell. 39: 267-74. PMID: 6094009
10. Expression of transferrin receptor on murine hematopoietic progenitors.  |  Lesley, J., et al. 1984. Cell Immunol. 83: 14-25. PMID: 6319033
11. Transferrin receptor expression as a marker of immature cycling thymocytes in the mouse.  |  Brekelmans, P., et al. 1994. Cell Immunol. 159: 331-9. PMID: 7994765
12. The transferrin receptor.  |  Testa, U., et al. 1993. Crit Rev Oncog. 4: 241-76. PMID: 8485201

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1) has 66 citations in a variety of scientific publications.

Primary antibodies like Anti-CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1) Specifications

Alternate Product Name: CD71

Product Clone Name: 3B8 2A1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD71

Epitope Species: human

Product Reactivity/Detection: CD71

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 66 citations in various scientific publications

Available Conjugates/Forms for CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin