CD68 Antibody (SPM130) recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF and IHC(P)

CD68 Antibody (SPM130): sc-52998

CD68 Antibody (SPM130) is rated 5.0 out of 5 by 5.
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Datasheets

    • CD68 Antibody (SPM130) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
    • raised against a subcellular fraction of human alveolar macrophages
    • recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See CD68 (KP1): sc-20060 for CD68 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using CD68 (SPM130): sc-52998 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Robust signal in WB in THP Robust signal in WB in THP-1 whole cell lysate. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from Good publishable data in IHC in rat kidney tissues Good publishable data in IHC in rat kidney tissues. -SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by from Superb immunoperoxidase macrophage staining Superb immunoperoxidase macrophage staining in formalin-fixed paraffin embedded human coronary artery tissue. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Worked in WB in K Worked in WB in K-562, U-937 and AML-193 whole cell lysates. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Strong staining in IF in rat immature erythroid Strong staining in IF in rat immature erythroid cells. -SCBT Publication Review
Date published: 2015-03-08
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