CD68 Antibody (KP1) recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD68 Antibody (KP1): sc-20060

CD68 Antibody (KP1) is rated 4.4 out of 5 by 42.
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Datasheets

    • Anti-CD68 Antibody (KP1) is a mouse monoclonal IgG1 κ CD68 antibody, cited in 120 publications, provided at 200 µg/ml
    • raised against a subcellular fraction of human alveolar macrophages
    • Anti-CD68 Antibody (KP1) is recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD68 Antibody (KP1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD68 (KP1): sc-20060.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for CD68 Antibody (KP1) for WB and IHC(P) applications. These reagents are now offered in bundles with CD68 Antibody (KP1) (see ordering information below).

What antigen retrieval method would you suggest for IHC-P?

Asked by: livlab
Thank you for your question. You can find our recommended method for antigen retrieval in our standart protocol, which is available under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support Europe
Date published: 2020-10-21

Were the THP-1 in the IF staining photos differentiated?

Asked by: ttam
Thank you for your question. No, this is a standard whole cell lysate that was used for this IF staining. The status of proteins in a cell lysate can vary, so localization of proteins can vary if depending on the status of the protein in the cells. We did not treat the cells in any special way.
Answered by: Tech Service
Date published: 2019-10-11

Hello! I am working on IHC-P project involving pigs heart tissue. would this antibody be suitable to work on pig tissues formalin fixed / paraffin embedded? Thanks

Asked by: Drzaid
Thank you for your question. We are not aware of any data or product citations using this antibody in pig samples.
Answered by: Technical Support
Date published: 2019-06-16

What kind of optimalisation (citrate/EDTA) does the antibody need for IHC-P on human brain tissue. I'd like to label microglia. Thanks

Asked by: doradavid11
Thank you for your question. We recommend using sodium citrate buffer (pH 6) with heat treatment for antigen retrieval with this antibody on FFPE. Please see our protocol for more details.
Answered by: Technical Support
Date published: 2019-01-31

When I use this antibody to run a flow cytometry experiment, should I apply a intracellular stain protocol or stain it in a surface staining way

Asked by: rhone
Thank you for your question. We recommend using a direct staining FCM protocol when using this mouse monoclonal antibody, CD68 (KP1): sc-20060. A link to our recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-06-01

What region of the protein does this antibody bind to? I see that it is reactive towards several species, so is it a conserved sequence?

Asked by: treedee21
Thank you for your question. CD68 Antibody (KP1) is a mouse monoclonal raised against a subcellular fraction of human alveolar macrophages. The exact epitope recognized by this antibody has not been determined.
Answered by: Technical Support
Date published: 2017-02-24

Should this AB react with cell lysate from the RAW cell line?

Asked by: treedee21
Thank you for your question. We do not have any evidence supporting expression of CD68 in the mouse RAW 264.7 cell line. We recommend using THP-1 (sc-2238), K-562 (sc-2203), AML-193 (sc-364182) and U-937 (sc-2239) whole cell lysates as positive control with this mouse monoclonal CD68 Antibody (KP1): sc-20060.
Answered by: Technical Support
Date published: 2017-01-26

Is it possible to use this antibody for immunofluorescence studies if I am working with mouse tissue?

Asked by: Trav11
Yes. Cross reactivity would be a concern only in tissues with high expected endogenous IgG content.
Answered by: Tech Service 4
Date published: 2017-01-06
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Rated 4 out of 5 by from Good signal in WB studies It works well in western blot studies using non-parenchymal cells (enriched in macrophages) isolated from rat livers
Date published: 2021-01-18
Rated 5 out of 5 by from Good performance Several months ago, we purchased the CD68 anti-body, and the histochemical result was very good.
Date published: 2020-10-09
Rated 5 out of 5 by from A very nice antibody. The antibody has high sensitivity and strong specificity, as well as its accurate positioning and clear staining background.
Date published: 2020-05-15
Rated 5 out of 5 by from Good performance One year ago, we purchased the antibody against CD68 (sc-20060) . The antibody works well.
Date published: 2019-08-26
Rated 5 out of 5 by from immunofluorescent staining on immunofluorescent staining, this antibody worked very well
Date published: 2019-04-16
Rated 3 out of 5 by from Good product This antibody worked for immunofluorescence staining with fixed frozen mouse tissue.
Date published: 2018-11-16
Rated 5 out of 5 by from Good AB! It works well on THP-1 differentiated macrophages in 1:100 dilution.
Date published: 2018-09-11
Rated 4 out of 5 by from Antibody review This antibody is good for immunoblotting at 1:500 dilution with mouse tissue lysate.
Date published: 2018-06-14
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