CD68 Antibody (3F103) recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD68 Antibody (3F103): sc-70761

CD68 Antibody (3F103) is rated 4.5 out of 5 by 13.
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Datasheets

    • CD68 Antibody (3F103) is a mouse monoclonal IgG1 κ, cited in 34 publications, provided at 200 µg/ml
    • raised against a subcellular fraction of human alveolar macrophages
    • recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD68 (3F103): sc-70761.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for CD68 Antibody (3F103) for WB and IHC(P) applications. This reagent is now offered in a bundle with CD68 Antibody (3F103) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What concentration for Flow cytometry is proper to use? It is suggested in the datasheet to use 1 ug/million cells, however, what concentration of Ab is recommended?

Asked by: Anonymous
Thank you for your question. This antibody, CD68 antibody, sc-70761, is provided as 200µg in 1ml, so if you need to use 1µg you will need to take 5µl of antibody solution. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-11-19

Which is the volume of the vial? Because it is referenced as 200 µg/ml but recommended concentrations are not in µg/ml

Asked by: VíctorSrX
Thank you for your question. CD68 Antibody (3F103) is a mouse monoclonal IgG1 provided at 200 µg in 1 mL volume.
Answered by: Technical Support
Date published: 2017-02-09

What is the best secondary to use with this antibody in Western Blot?

Asked by: Anna99
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17

Anyone knows which is the appropriate pH for Antigen Retrieval?

Asked by: VíctorSrX
Thank you for your question. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-11
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Rated 1 out of 5 by from Not good for WB; too faint even at 1:200 THP1 macrophages and Raw macrophages showed very faint bands to none when loaded15- 80ug proteins/well for WB; used 1:200 at Room temp after repeated use of this antibody for 2 days in cold.
Date published: 2018-12-20
Rated 4 out of 5 by from Good AB This antibody gave a good result in IHC on frozen human section in a dilution of 1:100.
Date published: 2018-04-03
Rated 5 out of 5 by from great for TAM detecting I am very glad to receive your prdoucts two weeks ago.we use this antibody to mark the monocytes cells isolated from the peripheral blood very well, which is very helpful to our research.
Date published: 2017-12-04
Rated 5 out of 5 by from CD 68 immunohistochemical staining for TAMs It worked for our tumor tissue biopsy sample. I got excellent result in immunohistochemical staining by using this CD68 antibody for our tumor-associated macrophage study.
Date published: 2017-12-04
Rated 3 out of 5 by from for WB analysis This antibody tried many times,at first no band.I tried different dilutions, different expose time,finally tried 1:200 and incubated 2hour at RT, however the band still not strong and the antibody conceration is a little low.
Date published: 2017-06-25
Rated 5 out of 5 by from Great CD68 antibody CD68 (3F103) works great for WB and IF. Specific IF staining of methanol-fixed HeLa cells showing membrane localization.
Date published: 2017-01-11
Rated 5 out of 5 by from CD 68 immunohistochemical staining for SCLC It worked for our FFPE format lymph node and lung tissue biopsy sample. We got excellent result in immunohistochemical staining by using this CD68 antibody for our macrophage phenotypic study.
Date published: 2016-12-13
Rated 5 out of 5 by from Highly recommendable antibody in IHC P Highly recommendable antibody in IHC P
Date published: 2015-12-07
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